Abstract: In this paper the model of metabolic pathway flux of FDP accumulation is established and the metabolic flux distributions are obtained at different times of FDP accumulation process.The experiments have showed that FDP accumulation in the medium produced by the present method is due to the redirected flux distributions in this metabolic pathway.The yield of FDP to glucose was only 16.1%.Meanwhile,the results also showed that such metabolic flux alterations at key branch points are often directly opposed by mechanisms for controlling enzyme activity,such as HK,PFK,PK, et al ,which have evolved to maintain flux distributions that are optimal for growth.So,in order to overproduce FDP with yeasts,enzyme amplification or other modifications of the product pathway must be done to alter the metabolic flux distributions at the key branch points.