Abstract: The effect of biological nutrient removal depends on the quantity and activity of nitrobacteria in activated sludge and biofilm. At medium temperature range，the conversion of ammonia nitrogen into nitro nitrogen is mainly conducted by the medium temperature nitrifying bacteria. While，most of the medium temperature nitrobacteria are hibernated bellow 10 ℃ and lose their metabolic ability below 4 ℃，which resulted in the insufficient treatment for waste water can not reach the standards. In this study，cold-adaptive nitrobacteria were isolated by cultivating the activated sludge in low temperature（≤10 ℃）to make the cold tolerance nitrobacteria become the dominant bacteria，with which low-temperature-resistant nitrobacteria were constructed by using entrapped bioaugmentation technique and their effects for nitrogen removal were investigated.

摘要： 生物脱氮除磷系统中硝化效果的好坏取决于活性污泥和生物膜中的硝化细菌的数量和活性。在中温条件下，将氨氮转化为硝酸盐氮的微生物主要是中温硝化细菌。但是大多数的中温硝化菌在温度降到10 ℃以下，进入休眠状态，当温度低于4 ℃时，大部分丧失新陈代谢能力，导致低温污水深度处理难以达标。本试验通过低温下（≤10 ℃）驯化培养活性污泥，使耐冷硝化菌在适宜的条件下生长成为优势菌种，并从中分离出高效耐冷硝化菌群，采用包埋固定化生物强化技术构建了耐低温硝化细菌，并对其脱氮效果进行了研究。