Abstract:

The in?uence of three different detoxi?cation methods（neutralization，overliming and Na₂SO₃  addition）on inhibitors were evaluated by using corn stover hydrolysate prepared with hydrothermal pretreatment（195 ℃，15 min）. Ethanol fermentability of detoxified corn stover hydrolysate was investigated by Pichia stipitis 58376. The results showed that all the employed detoxification methods resulted in a 41% reduction in average total furans and highest 28.4% reduction in total phenols. Fermentation performance was greatly enhanced by employed detoxification methods. Ethanol yield of 69.31% of the theoretical value based on reducing sugar was obtained by overliming. The corresponding ethanol concentration and volumetric productivity were 12.2 g/L and 0.056 g/（L?h）. Overliming was the most efficient detoxification method.

摘要：

利用湿热预处理（195 ℃，15 min）后的玉米秸秆水解液，考察了3种不同脱毒方法（中和法、饱和生石灰法和Na₂SO₃法）对水解液中的抑制剂的去除效果，研究了树干毕赤酵母（ Pichia stipitis 58376）对脱毒后的水解液酒精发酵情况。结果表明：玉米秸秆水解液经过3种方法脱毒处理后，醛类抑制剂（糠醛和5-羟甲基糠醛）平均减少41%，总酚类最高去除28.4%，酒精得率都得到明显提高。最佳的脱毒方法是饱和生石灰法，理论酒精得率达到69.31%，对应的酒精浓度和生产效率分别为12.2 g/L和0.056 g/(L·h)。饱和生石灰法是一种有效实用的脱毒方法。