Abstract:

For preparative applications in asymmetric reduction with alcohol dehydrogenase，three issues have to be considered：An appropriate enzyme，an efficient coenzyme-regenerating method and a suitable in vitro evolution technique. Enzyme is the source of effective biocatalyst. The native or recombinational alcohol dehydrogenases from Rhodococcus sp.，Lactobacillus sp.，Leifsonia sp. and thermophilic microbiology，and their catalysis were analyzed. The advantages and disadvantages of coenzyme-regeneration methods were discussed. The efficient coenzyme-regeneration can decrease costs for large-scale application. In vitro evolution technique can improve the activity，stability and selectivity of target enzyme. The final aim is to establish effective and green ways to obtain chiral drug intermediates by enzymatic synthesis.

摘要：

从醇脱氢酶在不对称还原制备手性醇化合物的应用出发，进行3个重要方面综述：酶分子——作为优质高效的生物催化剂，对红球菌属、乳酸菌属、赖氏菌属及嗜热微生物来源的天然或重组醇脱氢酶的催化作用进行探讨；辅酶的再生方法及优缺点分析——探索适合于特定催化反应的再生方法，以解决大规模应用时添加昂贵的辅酶带来的成本问题；体外进化技术——对目的酶活性、立体选择性与稳定性的提高。最终为酶催化制备医药及精细化工品中间体建立一条绿色高效经济的途径。