Abstract: The optimal fermentation time of Penicillium citrinum feruloyl esterase（PcFAE）was 60 h and its activity was 40.8 mU/mL when the Brewers’ Spent Grain（BSG）was used as the sole carbon source for Penicillium citrinum. One gram of alcohol-insoluble residue（AIR）of Brewers’spent grain（BSG）was processed by the solution of crude xylanase（XG180）（47.5 U/mL，15 mL）for 12 hours at the condition of the material-liquid ratio 1∶30（W/V），pH 5.0 and 45 ℃，respectively. And then the solution of crude PcFAE（40.8 mU/mL）was added and the sample was processed for another 12 h. The maximum release rate of Ferulic acid（FA）was 54.1% and the release amount of xylooligosaccharide（XOS）was 161 mg/g（BSG-AIR），respectively. The results of our experiments showed that the release rate of FA and XOS from BSG-AIR increased significantly as the PcFAE could coordinate with the xylanase and enzymolysis process was also conducive to the degradation of BSG.

Key words: feruloyl esterases；xylanase；brewers&rsquo, spent grain；biodegradation

摘要： 橘青霉以麦糟为唯一碳源培养时，阿魏酸酯酶的最佳发酵时间为60 h，其酶活力可达40.8 mU/mL。在pH值为5.0、45 ℃、料液比1∶30（g∶mL）条件下，取47.5 U/mL的木聚糖酶粗酶液15 mL，加入1.0 g 麦糟的乙醇不溶物，反应12 h后，加入40.8 U/mL的阿魏酸酯酶粗酶液15 mL再反应12 h，阿魏酸和低聚木糖释放率分别为54.1%和161 mg/g （麦糟的乙醇不溶物）。实验结果还表明，阿魏酸酯酶与木聚糖酶存在协同作用，能极大提高麦糟中阿魏酸及低聚木糖的释放率，有利于麦糟的降解。

关键词: 阿魏酸酯酶, 木聚糖酶, 麦糟, 生物降解