Abstract: The aqueous two-phase system of PEG-4000/K_3PO_4 is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically and werefermented in high expressivity.The effects of pH value and the concentrations of PEG and phos-phate on the partition behavious of γ-IFN and contaminating proteins have been investigated.Theresults show that the pH value is the most influential factor effecting the extraction yield of γ-IFN.Under a suitable condition,an effective separation with high yield of γ-IFN can be achieved.

Key words: aqueous two-phase extraction, interferon-γ

摘要： The aqueous two-phase system of PEG-4000/K_3PO_4 is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically and werefermented in high expressivity.The effects of pH value and the concentrations of PEG and phos-phate on the partition behavious of γ-IFN and contaminating proteins have been investigated.Theresults show that the pH value is the most influential factor effecting the extraction yield of γ-IFN.Under a suitable condition,an effective separation with high yield of γ-IFN can be achieved.

关键词: aqueous two-phase extraction;interferon-γ