Abstract: Sapindus saponin water extraction，hydrolyzed by Cellobiase and inoculated with Pseudomonas aeruginosa，was fermented to produce biosurfactant rhamnolipid. After fermentation，glucose was completely consumed，and glucose consumption rate was positively rated with loading of Pseudomonas aeruginosa. In the fermentation broth，additional soybean oil could promote formation of surfactant lipophilic group，and solution surface activity was further improved. After fermentation，the concentration of surface active material reached 50.8g/L，16.1% higher than that of the control experiment. The surface tension of the solution was significantly decreased. The critical micelle concentration of Sapindus saponin powder composite with 10% inoculum was reduced from 10g/L to 2.5g/L，and surface tension was 18.67% lower than Sapindus saponin powder without inoculum. The composite product had good foaming properties and high foam stability.

Key words: Sapindus mukurossi saponin water extraction, rhamnolipid, fermentation refining process, surface activity, foam property

摘要： 以无患子皂素水提水解液为底物，发酵生产生物表面活性剂，在精制无患子皂素的同时发酵联产生物表面活性剂鼠李糖脂。经铜绿假单胞菌发酵后发酵完成液中不含葡萄糖，葡萄糖的消耗速率与接种量成正相关，在发酵液中额外添加大豆油可促进表面活性剂亲油基团的生成，溶液表面活性进一步提高。发酵后溶液中表面活性物质浓度达到50.8g/L，比对照组提高了16.1%，溶液表面张力明显降低。接种10%的菌种发酵获得的无患子皂素复合产物干粉其临界胶束浓度由10g/L降低到2.5g/L，临界胶束浓度下的复合产物水溶液表面张力比未接种菌种的低18.67%，复合产物具有很好的起泡性能及更高的泡沫稳定性。

关键词: 无患子水提皂素液, 鼠李糖脂, 发酵精制, 表面活性, 泡沫性能