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STUDY ON THE TOXIC EFFECTS OF ORGANIC SOLVENTS ON YEASTS

Mei Lehe, Zheng Xiaodong and Yao Shanjing (Department of Chemical and Biochemical Engineering,Department of Food and Nutrition, Zhejiang University Hangzhou 310027)   

  • Online:2000-12-30 Published:2000-12-30

有机溶剂对不同种类酵母细胞毒害作用的初步研究

梅乐和,郑晓冬,姚善泾   

  1. 浙江大学化学工程与生物工程系!杭州310027,浙江大学食品与营养系!杭州310027,浙江大学化学工程与生物工程系!杭州310027

Abstract: The growth status of ten yeasts in the presence of several different polar organic solvents such as petroleum benzine,glycol methyl ether and polyethylene glycol octylphenyl ether and the living and growth of the yeasts in organic solvents with different logP value were examined.It is discovered that most of these yeasts can keep alive and grow in the presence of organic solvents. Especially the growth of Hansenula anomala in polyethylene glycol octylphenyl ethet, the CFU can reach 41%. These results show that the living and growth of the yeasts in the presence of organic solvents are strictly related with strain characters. Based on above results,the effects of the concentration of Mg2+ and the temperature on the living and growth of the yeasts in organic solvents were studied. For different yeasts the effect of Mg2+ is obviously different. The rising of temperature is unfavourable for the living and growth of the yeasts. Appropriate temperature is necessary for the yeasts to keep alive and grow in organic solvents and further bring into play the maximum catalysis efficiency in organic solvents.

摘要: 选择了石油醚、乙二醇甲醚、聚乙二醇辛基苯基酸等不同极性的有机溶剂,考察了10种酵母菌株在这些溶剂存在时的主长状况,考察了它们在具有不同的lgP的有机溶剂中的存活和生长情况,结果发现除个别菌株外都能在有机溶剂存在时存活并生长,尤以异常汉逊酵母在聚乙二醇辛基苯基醚存在时生长得最好,其存活率 CFU达 41%,表明在有机溶剂存在时酵母的存活和生长具有严格的菌种特性。在此基础上还研究了Mg2+浓度、温度对酵母菌株在有机溶剂中的存活和生长情况的影响,发现Mg2+浓度对不同的酵母菌株的存活和生长情况的影响存在着明显的差异,温度升高对酵母菌株的存活和生长不利,合适的温度对酵母菌株在有机溶剂中的存活和主长进而发挥酵母菌株最大催化效能是必须的。

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