CIESC Journal ›› 2022, Vol. 73 ›› Issue (7): 2790-2805.DOI: 10.11949/0438-1157.20220325

• Reviews and monographs • Previous Articles     Next Articles

Oxidative modification of plant natural products and microbial manufacturing

Xinzhe ZHANG1(),Wentao SUN2,Bo LYU1(),Chun LI1,2()   

  1. 1.Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China
    2.Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • Received:2022-03-02 Revised:2022-04-08 Online:2022-08-01 Published:2022-07-05
  • Contact: Bo LYU,Chun LI

植物天然产物氧化与微生物制造

张昕哲1(),孙文涛2,吕波1(),李春1,2()   

  1. 1.北京理工大学化学与化工学院生物化工研究所,北京 100081
    2.清华大学化学工程系生物化工研究所/ 工业生物催化教育部重点实验室,北京 100084
  • 通讯作者: 吕波,李春
  • 作者简介:张昕哲(1997—),男,硕士研究生,bit_zhangxinzhe@163.com
  • 基金资助:
    国家重点研发计划项目(2018YFA0901800);国家自然科学基金项目(21736002)

Abstract:

Oxidation reaction is the key reaction in the synthesis and modification of many plant natural products, oxidase is an indispensable biocatalyst for catalytic oxidation, and it is also an indispensable key enzyme in the synthesis of plant natural products by microorganisms. In this article, the oxidative modification of terpenoids, alkaloids, flavonoids and other plant natural products was reviewed, the oxidases in the synthesis of plant natural products were classified and introduced according to the differences of auxiliary groups, and the mechanism of the different auxiliary groups involved in the oxidation reaction was explained. In addition, this paper also introduces the difficulties of plant natural product oxidation in microbial synthesis and methods to improve the catalytic efficiency of oxidase. Finally, the future prospects of oxidase in the field of microbial synthesis of plant natural products in synthetic biology are prospected.

Key words: plant natural products, oxidative modification, oxidase, enzyme engineering

摘要:

在植物天然产物合成过程中,氧化反应是其中的关键反应,氧化酶是催化氧化反应不可或缺的生物催化剂,也是利用微生物合成植物天然产物过程中不可或缺的关键酶。介绍了萜类、生物碱、黄酮等植物天然产物骨架的氧化修饰,按照辅基的差异对合成植物天然产物过程中的氧化酶进行分类介绍,阐释了不同辅基参与氧化反应的机理。此外,还介绍了植物天然产物氧化过程在微生物合成过程中的难点,以及提高氧化酶催化效率的方法。最后,对未来合成生物学中氧化酶在微生物合成植物天然产物领域的前景进行了展望。

关键词: 植物天然产物, 氧化修饰, 氧化酶, 酶工程

CLC Number: