CIESC Journal ›› 2008, Vol. 59 ›› Issue (9): 2295-2301.
Previous Articles Next Articles
XU Hong, DOU Wenfang, XU Hongyu, ZHANG Xiaomei, RAO Zhiming, XU Zhenghong
Online:
Published:
许虹,窦文芳,许泓瑜,张晓梅,饶志明,许正宏
Abstract:
The effect of different oxygen supply levels by controlling flask capacity on the performance of producing L-arginine using Corynebacterium crenatum SYPA5-5 was investigated in the batch fermentation process.The analysis of fermentation kinetic models and metabolic flux indicated that the hexose monophosphate (HMP) pathway was much more active to produce nicotinamide adenine dinucleotide phosphate (NADPH) for bacterial growth at a high oxygen supply level.L-arginine production was also enhanced at a high oxygen supply level because of high glucose consumption.On the other hand, there was more metabolic flux from α-KG to Glu, the precursor of Arg.
Key words: L-精氨酸, 钝齿棒杆菌, 发酵, 供氧
L-精氨酸,
摘要: 以钝齿棒杆菌Corynebacterium crenatum SYPA5-5为研究菌株,通过不同摇瓶装液量试验,考察发酵过程中供氧对L-精氨酸合成的影响。作者分别采用发酵动力学模型和代谢流量分析对实验结果进行分析后发现:在发酵前期,高供氧使HMP途径更加活跃,生成大量NADPH促进菌体生长;高供氧控制同样利于L-精氨酸的合成,一方面是由于菌体的高葡萄糖摄入量,另一方面是由于有更多的流量从α-KG流向Arg的前体物质Glu。
关键词: L-精氨酸, 钝齿棒杆菌, 发酵, 供氧
XU Hong, DOU Wenfang, XU Hongyu, ZHANG Xiaomei, RAO Zhiming, XU Zhenghong. Effect of different oxygen supply levels on L-arginine batch fermentation process[J]. CIESC Journal, 2008, 59(9): 2295-2301.
许虹, 窦文芳, 许泓瑜, 张晓梅, 饶志明, 许正宏. 不同供氧水平对L-精氨酸分批发酵过程的影响 [J]. 化工学报, 2008, 59(9): 2295-2301.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://hgxb.cip.com.cn/EN/
https://hgxb.cip.com.cn/EN/Y2008/V59/I9/2295
Predictive control for anti-jamming on fermentation temperature control
Hybrid modeling for penicillin fermentation process