Abstract: Metabolic engineering is an approach to understanding and utilizing metabolic processes, by which metabolic networks and gene regulatory networks can be optimized or directly modified to enhance metabolites production or synthesize novel compounds.Development of systems biology and functional genomics in post-genomic era provides significant opportunities to construct microbial cell factories for metabolic engineering.The recent development of metabolic engineering in technology and application was reviewed, based on which several key issues were addressed and future perspectives were discussed.Finally, the strategy to develop metabolic engineering in China was proposed.

摘要： 代谢工程是一种理解并利用代谢过程的方法，其目的是优化或改变生物细胞中的代谢网络和表达调控网络，提高其代谢产物的产量或合成新的化合物.后基因组时代系统生物学和功能基因组学技术的发展，为构建能够实现特定目标的微生物细胞工厂提供了空前机遇.本文回顾代谢工程在技术和应用上的最新进展，讨论代谢工程中的关键问题和发展趋势，并提出中国在该领域的发展策略.