Abstract:

Eleven different macroporous adsorption resins were studied with respect to the static adsorption capacity and desorption ratio of vinblastine, vindoline and catharanthine.The best resin was AB-8 and the adsorption capacities of vindoline, catharanthine and vinblastine were 365.8, 254.2 and 24.8 mg·ml^-1, respectively.The optimum parameters of separation process by AB-8 resin were as follows: extraction solvent was sulfate dilution; pH value of the extraction was adjusted to 8 by ammonia; elute solvents were 20% alcohol to remove polar components, 50% alcohol (pH=4) to obtain the mono-indole alkaloids (vindoline and catharanthine) at 30℃ and 90% alcohol to obtain the dimeric-indole alkaloid (vinblastine).The results showed that the content of mono-indole alkaloids was above 50% and the purity of vinblastine was above 60%.

摘要：

通过对11种国产大孔树脂对文多灵、长春质碱和长春碱的静态吸附容量和解吸率等指标的考察，筛选出AB-8大孔吸附树脂作为分离长春花生物碱的载体，其对文多灵、长春质碱和长春碱吸附量分别为365.8、254.2、24.8 mg·ml^-1。利用大孔吸附树脂吸附分离长春花3种生物碱的过程为：长春花原料用稀硫酸溶液提取，提取液用氨水调节pH值为8，然后采用AB-8大孔吸附树脂柱吸附，用20%乙醇洗涤除去强极性成分，在30℃下用pH=4的50%乙醇解吸，得单吲哚生物碱文多灵和长春质碱，再用90%乙醇解吸，得双吲哚生物碱长春碱。单吲哚生物碱纯度可达50%以上，长春碱纯度达60%以上。