Abstract: The adsorption equilibria and kinetics of bovine serum albumin (BSA) on two hydrophobic adsorbents, Phenyl Sepharose FF low sub and Phenyl Sepharose FF high sub were investigated in the presence of different salt types and salt concentrations. The results indicated that an increasing salt concentration led to an enhanced adsorption capacity and a decreased dissociation constant, in particular in the salt system of sodium sulfate. Then, the effective pore diffusivity of BSA was predicted by fitting the pore diffusion model to the dynamic batch adsorption data. The results showed that effective pore diffusion coefficient increased with increasing salt concentration as well as ligand density.It was considered due to the enhanced contribution of surface diffusion to the interior mass transfer at high salt concentration and ligand density because of enhanced adsorption capacity under such conditions.

摘要： 研究了牛血清白蛋白在配基密度不同的两种疏水性吸附剂Phenyl Sepharose FF low sub和Phenyl Sepharose FF high sub上的吸附平衡和孔内传质动力学，重点考察了盐种类和浓度的影响.结果表明，Na2SO4溶液中盐浓度的增加导致蛋白质吸附容量的增大和解离常数的降低比(NH4)2SO4溶液更显著.利用孔扩散模型得到的有效扩散系数随盐浓度及配基密度的增大而提高，表明表面扩散作用对孔内传质的贡献随吸附容量提高而增大.