Determination of competitive adsorption isotherms of tryptophan enantiomers by inverse method
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CHENG Ming;LI Shuang;ZHANG Fengbao;ZHANG Guoliang
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逆向法确定色氨酸对映体竞争型吸附等温线
程明;李爽;张凤宝;张国亮
天津大学化工学院,天津 300072
Abstract: Competitive adsorption isotherm data for two enantiomers of tryptophan, in which the stationary phase was silica-immobilized bovine serum albumin(BSA) and the mobile phase was a phosphate buffer,were measured by using the inverse method.The competitive bi-Langmuir isotherm model(Fisher coefficient is 1769.07, R2=0.9997) and equilibrium-dispersive model were selected by comparison of the experimental results and the interaction mechanism.The corresponding parameters were obtained by simulating the chromatogram of racemic mixture at 2×10-4 mol8226;L-1 and 10-3 mol8226;L-1.The results showed good agreement between the isotherm determined by the inverse method and the adsorption data (Fisher coefficient is 119.32, R2=0.9973) which confirmed the validity of the model selected.
CHENG Ming;LI Shuang;ZHANG Fengbao;ZHANG Guoliang.
程明;李爽;张凤宝;张国亮 .
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https://hgxb.cip.com.cn/EN/Y2006/V57/I5/1177