Abstract: Factorial design and response surface techniques were used to design and optimize the process to increase P450 BM-3 activity produced by gene engineering strain.Initially, Plackett-Burman design was used to evaluate the process variables which were relevant to the production of P450 BM-3.Four statistically significant parameters for response were selected and utilized in order to optimize the process.With the 416C model of hybrid design, response surfaces were generated, and P450 BM-3 activity was increased to 57.9×10^-3Uml^-1 from 37.6×10^-3Uml^-1 by the best combination of the physicochemical parameters at optimal levels of 0.12 mgL^-1 FeCl₃, inoculum volume of 2.1%, induction at OD₅₇₈=1.1, and 6 h of induction.

摘要： 采用快速有效的数学统计方法对工程菌产细胞色素P450 BM-3的发酵条件进行了优化.首先利用Plackett-Burman设计从众多影响产P450 BM-3的因素中筛选出影响较大的4个因素：FeCl₃含量、诱导时机、诱导时间和接种量.在此基础上再利用响应面法中的杂合设计进行优化，通过拟合得到响应曲面函数，获得了最佳的实验条件.在该实验条件下，P450 BM-3酶活从37.6×10^-3U•ml^-1提高到57.9×10^-3U•ml^-1.