Abstract:

Based on the technology of micro-fluidics digitalization, a new method of preparing cell microcapsules is proposed.The method has the advantage of simple structure, low cost, and mild preparation conditions.A pulse driving and controlling system of micro-fluidics was designed based on electromagnet actuator.Pulse inertial force with controllable size and frequency was applied to a micro-nozzle and sodium alginate cell suspension inside the nozzle through controlling the reciprocating magnetic core, which bumped a vibrating object, to which the micro-nozzle was fixed.By using this method, a single fish roe cell (about 900μm）was injected steadily and cell microcapsules were prepared stably.Optimum parameters for stable preparation were determined.The parameters included clamping angle of micro-nozzle θ, voltage amplitude U, frequency f and length of liquid column L.The prepared fish roe cell microcapsules had uniform wall-thickness, regular shape and good monodispersity.The size of microcapsules could be changed by choosing micro-nozzles with different inner diameters.The viability of microencapsulated cells was indirectly measured by trypan blue stain solution and the result was approximately 90%.

Key words: 细胞, 微胶囊, 微流体数字化, 液滴喷射

摘要：

基于微流体数字化技术，提出了一种结构简单、成本低、制备条件温和的细胞微胶囊制备方法。设计了以电磁铁为作动器的微流体脉冲驱动控制系统，通过控制电磁铁铁芯往复运动撞击固定有微喷嘴的振动体，为微喷嘴及其内部的海藻酸钠细胞悬浮液提供频率和大小可控的脉冲惯性力，实现了直径约为900 μm的单个鱼卵细胞的稳定喷射和鱼卵细胞微胶囊的稳定制备。给出了稳定制备时系统最优参量组合（微喷嘴装夹角度θ、驱动电压U、驱动频率f和液柱长度L）的确定方法。制备出的鱼卵细胞微胶囊壁厚均匀、形状规则且具有良好的单分散性。通过改变微喷嘴出口内径的大小可以实现对微胶囊粒径的控制，使用台盼蓝染色液间接测量微囊化后的鱼卵细胞存活率约为90%。

关键词: 细胞, 微胶囊, 微流体数字化, 液滴喷射