Abstract: Glycerol production by reusing free Candida glycerinogenes cells in repeated batch fermentation was studied.The results showed that the suitable concentration of KH2PO4 in the re-inoculation medium was 0.1 g·L-1, lower than KH2PO4 of 0.4 g·L-1 in the first fermentation medium.When the residual glucose concentration decreased to 10 g·L-1, yeast cells from sterile centrifugation with the activity high enough for the next cycle could be directly recycled for the next batch fermentation.After 15 cycles of re-inoculation, average glycerol concentration, yield and productivity were 138.69 g·L-1, 60.17% and 2.31 g·L-1·h-1, with an increase of 15.74%, 15.48% and 39.16%, respectively, compared with these in the first batch fermentation.Although yeast cells decayed severely in the fifteenth cycle, 12—14 cycles of repeated batch fermentation were possible.

摘要： 研究了产甘油假丝酵母细胞回用生产甘油的反复分批发酵。实验结果表明：第一级发酵培养基KH2PO4浓度为0.4 g·L-1，回用培养基的最适KH2PO4浓度为0.1 g·L-1，当上一批次发酵液中葡萄糖浓度降至10 g·L-1以下时，酵母细胞不经洗涤即可回用；经过15个批次的反复分批发酵过程，甘油的平均产量、平均得率和平均生产能力分别达到138.69 g·L-1、60.17%和2.31 g·L-1·h-1，分别比第一级发酵结果增加了15.74%、15.48%和39.16%；但回用至第15次时，菌体出现严重衰退，因此回用周期以12～14次为宜。