Abstract: Two level factorials designs of Plackett-Burman were constructed to select the Pseudomonas W20 fermentation medium components of α-chloropropionic acid dehalogenases.Three important components were urea, glucose and KH2PO4.The optimized levels of these factors were determined by the response surface analysis. The results showed that the optimum concentrations of urea,glucose and KH2PO4 were 1.19 g·L-1,18.4 g·L-1 and 1.30 g·L-1 respectively.The enzyme activity increased by 23.77% under the optimized condition.

摘要： 通过Plackett-Burman设计和响应面分析方法对Pseudomonas W20菌株产脱卤酶的培养基组成进行优化。得出影响产酶的3个重要的培养基成分为：尿素、葡萄糖和KH2PO4，且其最适浓度分别为1.19、18.4 g·L-1和1.30 g·L-1。此时脱卤酶活力达到了10.57 U·（g干菌体）-1，比优化前提高了23.77%。