Abstract: In the first part of this paper, the GET composite copolymer with pendant epoxy groups were prepared. It was reacted with diethylamine, forming an anion-exchange chromatographic medium DEAE-GET. The effects of liquid viscosity, flow velocity on expansion characteristics and liquid mixing performance of DEAE-GET in expanded bed were studied. Its expansion characteristics in STREAMLINE 25 column were found to obey the Richardson-Zaki equation. The increase of liquid viscosity, flow velocity resulted in the increase of axial mixing coefficient. In the range of flow velocity 100—500 cm•h^-1, the low value of axial mixing coefficient in expanded bed (10^-6—10^-5m²•s^-1) indicated that the liquid flow through the expanded bed was close to a plug flow profile. The maximum static adsorption capacity for BSA was found to be 43.0 mg•ml^-1.The breakthrough curves for BSA adsorption on DEAE-GET and STREAMLINE DEAE in packed bed and expanded bed were compared,showing for each adsorbent they were close to each other.The maximum adsorption capacities on DEAE-GET in packed bed and expanded bed were 28.0 mg•ml^-1 and 26.2 mg•ml^-1 respectively.

Key words:

扩张床, 吸附, 牛血清白蛋白

摘要： 利用GET共聚物骨架结构中的环氧基与二乙胺反应得到的阴离子交换层析介质DEAE-GET，考察了该介质在扩张床STREAMLINE 25中不同黏度和流速对扩张性能和流体混合性能的影响，结果表明DEAE-GET在STREAMLINE 25中的扩张行为服从Richardson-Zaki方程.在研究的范围内，随着流体黏度和流速（100～500 cm•h^-1）的增加，理论塔板数和Bodenstein数逐渐减小，轴向混合系数逐渐增加；扩张床中的轴向混合系数很小（10^-6～10^-5 m²•s^-1），表明柱内流体以近似平推流流动.在静态条件下DEAE-GET对牛血清白蛋白（BSA）的饱和吸附量为43.0 mg•ml^-1.同时比较了DEAE-GET与STREAMLINE DEAE分别在固定床和扩张床中对BSA的穿透曲线，发现都比较接近，DEAE-GET在固定床和扩张床中对BSA的最大吸附量分别为28.0 mg•ml^-1和26.2 mg•ml^-1.

关键词:

扩张床, 吸附, 牛血清白蛋白