Abstract: In this paper the model of metabolic pathway flux of FDP accumulation is established and the metabolic flux distributions are obtained at different times of FDP accumulation process.The experiments have showed that FDP accumulation in the medium produced by the present method is due to the redirected flux distributions in this metabolic pathway.The yield of FDP to glucose was only 16.1%.Meanwhile,the results also showed that such metabolic flux alterations at key branch points are often directly opposed by mechanisms for controlling enzyme activity,such as HK,PFK,PK, et al ,which have evolved to maintain flux distributions that are optimal for growth.So,in order to overproduce FDP with yeasts,enzyme amplification or other modifications of the product pathway must be done to alter the metabolic flux distributions at the key branch points.

摘要： 首次建立了 1 ,6-二磷酸果糖 (FDP)累积的代谢途径流量模型 .应用该模型求解FDP累积过程中不同时期的代谢流量分配 ,详细分析了各时期代谢流量分配发生改变的原因 ,并探讨FDP累积的机理 .细胞膜通透性的改变是FDP积累于胞外的前提条件 ;而代谢网络中HK、PFK、Ald和PK等酶活的调节使主要结点处代谢流量分配发生重大改变 ,则是FDP在胞外大量积累的关键 .