Abstract:

The traditional synthesis of chemicals by esterification or transesterification requires relatively harsh conditions of heat, acid or alkali. Lipase-catalyzed processes are receiving more attentions as a promising alternative approach in the synthesis of chemicals because of its improving reaction efficiency, high selectivity, mild conditions and environment-friendly trait. Our group has developed a new lipase from Candida sp.,for enzymatic synthesis of esters. The lipase from Candida sp.99-125 exhibited excellent activity and stability for esterification and transesterification in non-aqueous media. Production of the lipase and various examples of its applications in enzymatic synthesis of chemicals, such as medium- and long-chain fatty acid esters, dibasic acid esters, vitamin A palmitate, biodiesel and chiral intermediates, were presented in this paper.

摘要：

传统的酯化或转酯化产品的合成通常需要高温、强酸、强碱等相对苛刻的条件，脂肪酶由于其生物催化过程具有高效、高选择性、条件温和和环境友好等特点，在化学品的合成中越来越受到人们的关注。本课题组开发了一种可以用于酯类合成的新脂肪酶，并且实现了该酶的工业化生产。来源于Candida sp.99-125的脂肪酶在非水相中对酯化和转酯化反应具有高效的催化活性和稳定性。本文介绍了该脂肪酶的发酵生产及其在中长链脂肪酸酯、二元酸酯、维生素A棕榈酸酯、手性化合物以及生物柴油等多种化学品的合成中的应用。