铜抗性菌株的筛选及其对Cu2+的吸附性能

doi:10.3969/j.issn.0438-1157.2013.09.041

化工学报 ›› 2013, Vol. 64 ›› Issue (9): 3381-3389.DOI: 10.3969/j.issn.0438-1157.2013.09.041

铜抗性菌株的筛选及其对Cu²⁺的吸附性能

李兰松^1,2, 杨永珍^2,3, 贾虎生², 杨利擎^1,2, 曹秋芬⁴, 刘旭光^1,2

1. 太原理工大学化学化工学院, 山西太原 030024;
2. 新材料界面科学与工程教育部重点实验室, 山西太原 030024;
3. 太原理工大学新材料工程技术研究中心, 山西太原 030024;
4. 山西省农业科学院生物技术研究中心, 山西太原 030031

收稿日期:2013-01-17 修回日期:2013-04-30 出版日期:2013-09-05 发布日期:2013-09-05
通讯作者: 刘旭光
作者简介:李兰松(1980- ),女,博士研究生,讲师。
基金资助:
长江学者与创新团队发展计划项目(IRT0972);国家自然科学基金项目(21176169);太原理工大学2012年校青年基金项目(2012L019)。

Isolation and Cu²⁺ biosorption of metal-tolerant bacterium

LI Lansong^1,2, YANG Yongzhen^2,3, JIA Husheng², YANG Liqing^1,2, CAO Qiufen⁴, LIU Xuguang^1,2

1. School of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China;
2. Key Laboratory of Interface Science and Engineering in Advanced Materials, Ministry of Education, Taiyuan 030024, Shanxi, China;
3. Research Center on Advanced Materials Science and Technology, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China;
4. Agricultural Biotechnology Research Center of Shanxi Province, Taiyuan 030031, Shanxi, China

Received:2013-01-17 Revised:2013-04-30 Online:2013-09-05 Published:2013-09-05
Supported by:
supported by Changjiang Scholar and Innovative Research Team in University(IRT0972),the National Natural Science Foundation of China(21176169)and Youth Foundation of Taiyuan University of Technology(2012L019).

摘要/Abstract

摘要： 利用选择性培养基分离出铜抗性细菌BX,经鉴定该菌为产碱普罗威登斯菌(Providencia alcalifaciens)。考察了细菌BX对环境的适应性,讨论了pH、初始Cu²⁺浓度等对其吸附铜离子的影响,分析了吸附过程的动力学及等温吸附特性,并以多孔陶瓷为载体对其进行固定化。结果表明,该菌对Cu²⁺和NaCl的抗性浓度分别为7 mmol·L^-1和7.5%,可生长于pH4.0～11.0、15～50℃的环境中;其最佳吸附条件为pH5.5、温度30℃、起始Cu²⁺浓度100 mg·L^-1,在该条件下,Cu²⁺吸附率达85.84%,吸附量为128.74 mg·g^-1;其对Cu²⁺的吸附符合准二级动力学方程和Freundlich等温吸附模型;采用曝气挂膜法将细菌固定于多孔陶瓷上,形成的菌膜对50 mg·L^-1铜离子的吸附率达92.53%。表明细菌BX对Cu²⁺有较强的吸附能力,对含Cu²⁺废水的处理具有良好的应用前景。

关键词: 铜离子, 废水处理, 产碱普罗威登斯菌, 生物吸附

Abstract: The metal-tolerant bacterium strain BX,was isolated from polluted soils.The result of 16S rDNA sequence analysis indicated that the bacterium was Providencia alcalifaciens.The adaptability of bacterium BX to surroundings was discussed.The effects of the adsorption conditions,such as pH and temperature on the adsorption capacity by the bacterium BX from aqueous solutions were also investigated. Kinetic and isothermal study of BX toward Cu²⁺ was carried out.Immobilization was performed taking porous ceramics as carrier.The results showed that the bacterium BX could tolerant 7 mmol·L^-1 of Cu²⁺ and 7.5% of NaCl,and its growth was observed at conditions of pH4.0—11.0 and 15—50℃.The bacterium BX removed 85.84% of Cu²⁺ after 240 min of incubation in a solution containing 100 mg·L^-1 of Cu²⁺ at pH5.5,and the adsorption capacity of Cu²⁺ reached 128.74 mg·g^-1(dry bacterium).Kinetic study showed that the pseudo-second-order kinetic model fitted well with the experimental data whereas intraparticle diffusion could not be ignored,suggesting a chemical reaction mechanism.This would indicate that the adsorption of Cu²⁺ is a complex mix of surface adsorption occurring in the boundary layer of the bacterial particle and intraparticle diffusion.The isotherm biosorption of BX toward Cu²⁺was in accordance with the Freundlich model,suggesting that the adsorption process followed a monolayer sorption.After the formation of biofilm on the porous ceramics,the adsorption rate of BX on 50 mg·L^-1 copper ion solution reached 92.53%.The experimental results demonstrated the potential application of the tested bacterium BX in the treatment of heavy metal wastewater for the strong adsorption under a wide range of environmental conditions.

Key words: copper ion, wastewater treatment, Providencia alcalifaciens, biosorption

中图分类号:

X703

李兰松, 杨永珍, 贾虎生, 杨利擎, 曹秋芬, 刘旭光. 铜抗性菌株的筛选及其对Cu²⁺的吸附性能[J]. 化工学报, 2013, 64(9): 3381-3389.

LI Lansong, YANG Yongzhen, JIA Husheng, YANG Liqing, CAO Qiufen, LIU Xuguang. Isolation and Cu²⁺ biosorption of metal-tolerant bacterium[J]. CIESC Journal, 2013, 64(9): 3381-3389.

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铜抗性菌株的筛选及其对Cu²⁺的吸附性能

Isolation and Cu²⁺ biosorption of metal-tolerant bacterium

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