化工学报 ›› 2013, Vol. 64 ›› Issue (9): 3404-3411.DOI: 10.3969/j.issn.0438-1157.2013.09.044

• 能源和环境工程 • 上一篇    下一篇

AD-MFC中甲醇与硝酸盐的偶合过程与作用机制

张吉强1, 郑平1, 张萌1, 厉巍1, 陈慧1, 蔡琛1, 谢作甫1,2   

  1. 1. 浙江大学环境工程系, 浙江 杭州 310058;
    2. 浙江科技学院, 浙江 杭州 310023
  • 收稿日期:2013-01-06 修回日期:2013-02-28 出版日期:2013-09-05 发布日期:2013-09-05
  • 通讯作者: 郑平
  • 作者简介:张吉强(1986- ),男,博士研究生。
  • 基金资助:

    国家自然科学基金项目(31070110);高等学校博士学科点专项科研基金(20110101110078);浙江省自然科学基金重点项目(Z5110094);浙江省自然科学基金项目(Y5110266)。

Coupling process and mechanism of methanol oxidation and nitrate reduction in an anodic denitrification microbial fuel cell(AD-MFC)

ZHANG Jiqiang1, ZHENG Ping1, ZHANG Meng1, LI Wei1, CHEN Hui1, CAI Chen1, XIE Zuofu1,2   

  1. 1. Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, Zhejiang, China;
    2. Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China
  • Received:2013-01-06 Revised:2013-02-28 Online:2013-09-05 Published:2013-09-05
  • Supported by:

    supported by the National Natural Science Foundation of China(31070110),the Specialized Research Fund for the Doctoral Program of Higher Education of China(20110101110078),the Natural Science Foundation of Zhejiang Province(Z5110094,Y5110266).

摘要: 在双室微生物燃料电池(MFC)阳极内接种反硝化细菌富集培养物,同时加入硝酸盐和甲醇,构建了阳极反硝化微生物燃料电池(AD-MFC),并以批式操作研究了AD-MFC的反硝化产电性能。试验结果表明,在初始硝氮浓度为(100.22±0.62)mg·L-1,COD浓度为(500.40±1.67)mg·L-1的条件下,AD-MFC的最大容积NO3--N和COD去除速率分别达到0.31 kg N·m-3·d-1和1.06 kg COD·m-3·d-1,最大电压达到(602.80±5.42)mV,相应最大功率密度为(908.42±0.07)mW·m-3。AD-MFC的产电过程是甲醇氧化与硝酸盐还原的偶合过程,电压变化与反硝化作用密切相关,可用于指示反硝化进程。AD-MFC的电压曲线呈现降低-升高-再降低的三阶段特性,其原因是反硝化作用、甲醇降解作用和细胞水解发酵作用依次成为阳极液中的主导反应。

关键词: 微生物燃料电池, 反硝化作用, 产电性能, 脱氮性能

Abstract: An anodic denitrification MFC(AD-MFC)was constructed by inoculating denitrifying bacteria enrichment culture into anode chamber of a dual-chamber MFC,and its capacity of denitrification and electricity production was investigated with methanol as electron donor and nitrate as acceptor in a batch cultivation mode.When initial nitrate and COD concentration were (100.22±0.62)mg·L-1 and (500.40±1.67)mg·L-1,the volumetric nitrogen and COD removal rates reached 0.31 kg N·m-3·d-1 and 1.06 kg COD·m-3·d-1,respectively,coupled with the maximum voltage of (602.80±5.42)mV and the maximum power density of (908.42±0.07)mW·m-3.The results demonstrated that power generation in the AD-MFC was due to a coupling of methanol oxidation and nitrate reduction,and voltage changes were closely related to denitrification,indicating the course of denitrification.Voltage curves of the AD-MFC showed three-phase characteristics of descend phase,ascend phase and redescend phase,which could be ascribed to the succession of denitrification,methanol degradation and cell hydrolysate fermentation as dominant reaction in the anolyte.

Key words: microbial fuel cell, biological denitrification, electricity generation, nitrogen removal

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