Abstract: Concentrating eicosapentaenoic acid ( EPA) from fish oil with supercritical CO2 has been carried out using a laboratory rectification column of 9. 7mm internal diameter and 1. 1m effective height. To improve the supercritical fluid -liquid contact, a scourer rotor was used in the extraction cell, and the column was packed with Dixon ring. Also, to level up the separation ability, the extraction pressure was step -by -step programmed, and the column temperature gradient from bottom to top was kept at 35 to 80℃. Under such conditions, the maximum concentration of EPA was 90%, although plenty of components 20 : 4 was contained in the experimental feedstock. The work presented shows the possibility of purification of EPA from fish oil with supercritical CO2.