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透明颤菌血红蛋白基因和λ噬菌体裂解基因在生产PHB重组大肠杆菌中的同时表达

于慧敏a; 史悦a; 尹进a; 沈忠耀a; 杨胜利b   

  1. a Department of Chemical Engineering, Tsinghual Jniversity, Beijing 100084, China
    b Shanghai ResearchCenter of Biotechnology, Academic Sinica, Shanghai 200233, China
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-12-28 发布日期:2001-12-28
  • 通讯作者: 于慧敏

Simultaneous Expression of Vitreoscilla Globin Gene and Lytic Genes of Phage γ in a Novel
Recombinant Eseheriehia Coli Used for Productionof PHB

YU Huimina; SHI Yuea; YIN Jina; SHEN Zhongyaoa; YANG Shenglib   

  1. a Department of Chemical Engineering, Tsinghual Jniversity, Beijing 100084, China
    b Shanghai ResearchCenter of Biotechnology, Academic Sinica, Shanghai 200233, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-12-28 Published:2001-12-28
  • Contact: YU Huimin

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摘要： Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage γ with S amber mutation
(S^-RRz) and poly(fl-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a
same Escherichia coil cell,simultaneously or respectively. Six novel strains containing
phbCAB and vgb with or without lyticgenes were constructed. Strain VG1 (pTU14), in which
vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in
cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB
without S- RRz were not better than that of strains harbored phbCAB only. The simultaneous
expression of vgb and S-RRz in the recombinant VG1 (pTU14) showed a great potential for
low-cost production of PHB.

关键词: 透明颤菌血红蛋白基因;γ噬菌体裂解基因;化工生产;PHB;细胞重组;大肠杆菌;同时表达;聚(β- 羟基丁酸酯);生物合成

Abstract: Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage γ with S amber mutation
(S^-RRz) and poly(fl-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a
same Escherichia coil cell,simultaneously or respectively. Six novel strains containing
phbCAB and vgb with or without lyticgenes were constructed. Strain VG1 (pTU14), in which
vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in
cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB
without S- RRz were not better than that of strains harbored phbCAB only. The simultaneous
expression of vgb and S-RRz in the recombinant VG1 (pTU14) showed a great potential for
low-cost production of PHB.

Key words: Vitreoscilla hemoglobin, lytic genes of phage λ, poly (β-hydroxybutyrate)

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