关键词:

L-天门冬酰胺酶, 酶释放, 大肠杆菌, 双水相系统, 磷酸氢二钾, 聚氧乙烯辛基苯基醚

Abstract: A method to release L-asparaginase(EC3.5.1.1) from Escherichia coli ATCC 11303 cells using an aqueous two-phase system was proposed. The system was composed of K₂HPO₄ and polyoxyethylene octyl phenyl ether (POOPE). This method combined enzyme release with enzyme purification.The influences of POOPE concentration, K₂HPO₄ concentration,E. coli cell concentration,pH and treatment time on the release of L-asparaginase were investigated.Under suitable conditions,over 90% of L-asparaginase in cells was released into surrounding medium.Electron micrography indicated that the chemical treatment altered the inner structure of E.coli cells significantly.

Key words:

L-天门冬酰胺酶, 酶释放, 大肠杆菌, 双水相系统, 磷酸氢二钾, 聚氧乙烯辛基苯基醚