液固层析辅助蛋白质从变性态恢复到活性态

化工学报 ›› 2006, Vol. 57 ›› Issue (8): 1802-1809.

液固层析辅助蛋白质从变性态恢复到活性态

陈净;刘永东;李京京;苏志国

中国科学院过程工程研究所生化工程国家重点实验室，北京 100080;中国科学院研究生院，北京 100080

出版日期:2006-08-25 发布日期:2006-08-25

Refolding of protein from denatured state to active conformation by liquid chromatographic processes

CHEN Jing;LIU Yongdong;LI Jingjing;SU Zhiguo

Online:2006-08-25 Published:2006-08-25

摘要/Abstract

摘要：

层析是化工中分离纯化一些价格昂贵的精细化工产品的常用手段，而液固层析因其温和、快捷、高效等优点已经成为蛋白质纯化过程中必不可少的工具.近年来层析技术的一个拓展是用于辅助蛋白质从变性态恢复到活性态，即所谓的复性，取得了令人瞩目的进展.液固层析辅助蛋白质复性可在高蛋白浓度下操作，适用于大规模生产，同时还能使目标蛋白得到部分纯化.对近年来发展的利用液固层析辅助蛋白质复性的方法做了归纳，包括凝胶过滤层析、离子交换层析、疏水相互作用层析、亲和层析和固定化的折叠催化剂及人工分子伴侣辅助蛋白质复性的方法，并对各自的优缺点和适用范围进行了比较分析.

关键词:

蛋白质, 液固层析, 再折叠, 复性

Abstract:

Chromatography is commonly used in separating expensive fine chemical products.Liquid chromatographic procedure is the essential tool in protein purification because of its mild condition, quick and effective separation.In recent years, chromatography has been gradually used in protein refolding from denatured state to biologically active conformation.In the liquid chromatography-based protein refolding procedure,a high yield could be achieved at high protein concentrations.Partial purification of protein could also be achieved simultaneously.The recent development of liquid chromatography-based protein refolding is reviewed in this paper.The principles, advantages, disadvantages and applications are discussed with respect to various chromatographic refolding processes, including size exclusion chromatography, ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography with immobilized refolding catalyst and artificial chaperone.

Key words:

蛋白质, 液固层析, 再折叠, 复性

陈净, 刘永东, 李京京, 苏志国. 液固层析辅助蛋白质从变性态恢复到活性态 [J]. 化工学报, 2006, 57(8): 1802-1809.

CHEN Jing, LIU Yongdong, LI Jingjing, SU Zhiguo. Refolding of protein from denatured state to active conformation by liquid chromatographic processes[J]. CIESC Journal, 2006, 57(8): 1802-1809.

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