关键词:

固定化β-葡萄糖苷酶, 两相体系, 大豆异黄酮, 大豆苷元, 染料木素

Abstract:

β-Glucosidase was immobilized on chitosan beads by cross-linking with glutaraldehyde, and was characterized.The optimum pH and temperature were 5 and 40℃, respectively.Soybean isoflavone was hydrolyzed in a two-phase system containing ethyl acetate and aqueous solution (pH=5).In this system，immobilized enzyme was more stable compared to free enzyme, and faster reaction rates and higher yields were obtained compared to the corresponding aqueous solution.The yields of the two main hydrolysis products (daidzein and genistein) of soybean isoflavone were both around 70%.

Key words:

固定化β-葡萄糖苷酶, 两相体系, 大豆异黄酮, 大豆苷元, 染料木素