CIESC Journal

• 生物化工 • 上一篇    下一篇

固定化HRP/H2O2/ACAC酶促体系引发下降解淀粉接枝丙烯酰胺共聚物的合成与表征

闫小亮,吕生华,侯明明,弓 瑞   

  1. 陕西科技大学资源与环境学院,陕西 西安 710021
  • 出版日期:2011-12-05 发布日期:2012-03-02

Synthesis and characterization of graft copolymer of degraded starch and acrylamide initiated by immobilized HRP/H2O2/ACAC catalyzed system

YAN Xiaoliang,Lü Shenghua,HOU Mingming,GONG Rui   

  1. College of Resource and Environment,Shaanxi University of Science & Technology,Xi’an 710021,Shannxi,China
  • Online:2011-12-05 Published:2012-03-02

摘要:

以壳聚糖为载体、戊二醛为交联剂制备固定化辣根过氧化物酶(HRP),采用固定化HRP/H2O2/乙酰丙酮(ACAC)酶促体系制备降解淀粉-丙烯酰胺接枝共聚物(St-g-PAM),通过红外光谱、紫外光谱、核磁共振和电镜扫描等手段对接枝共聚产物进行了结构分析。结果表明,丙烯酰胺成功接枝在降解淀粉上。共聚物用做皮革复鞣剂进行了应用实验,应用结果表明复鞣后的革柔软、纤维分散好和选择填充性强。

Abstract:

Horseradish peroxidaseHRPwas immobilized on the surface of chitosan via glutaraldehyde crosslinking. Then a graft copolymer of degraded starch and acrylamide was synthesized by the initiation of enzyme catalysis system composed of chitosan-immobilized HRP/H2O2/acetylacetoneACAC. The structure of the copolymer was analyzed by FTIRUVNMR and SEM. The results indicated that polyacrylamidePAMwas successfully grafted onto the degraded starch. Thenthe copolymer was applied as a retanning agent. The applied results showed that the retanned leather had the merits of softnessgood dispersion of fiber and strong selecting filling properties.