Abstract:

Low specific activity，high enzymatic loading and easy acitivity losing of cellulose enzyme have become bottlenecks for bioprocessing the lignocellulosic biomass into bioenergy and bioproducts. In this work，non-ionic surfactants were added to reduce the enzymatic loadings. Its effect on the hydrolysis of lignocelluloses was also studied. Experimental results showed that the non-ionic surfactant helped to enhance the hydrolysis of lignocellulose with 0.5% addition. With the addition of Tween-80 and PEG6000，Glucose produced from enzymatic hydrolysis of atmospheric glycerol pretreated wheat straw increased approximately 20%. The non-ionic surfactant also had a positive impact on the enzymatic hydrolysis of cellulosic biomass with less lignin and even no lignin. The glucose outputs from the filter paper and avicel increased almost by 90% and 70%—80%，respectively. The addition of non-ionic surfactants provided a higher diffusion coefficient k for enzymatic hydrolysis system，increased reaction efficiency in heterogeneous phase，and made a significantly lower K_m in enzymatic reaction dynamics，therefore remarkably enhanced the affinity of substrates on cellulase.