沙雷氏菌 sp.BK-98发酵生产2-酮基-D-葡萄糖酸的工艺优化及动力学研究

化工学报 ›› 2011, Vol. 62 ›› Issue (5): 1371-1376.

沙雷氏菌 sp.BK-98发酵生产2-酮基-D-葡萄糖酸的工艺优化及动力学研究

张炜，谢志鹏，罗玮，张建国

1浙江大学生物化学研究所，浙江杭州 310058；2浙江大学生物工程研究所，浙江杭州 310027

出版日期:2011-05-05 发布日期:2011-05-05

Fermentation process optimization and kinetics studies of 2-keto-D-gluconic acid production by Serratia sp.BK-98

ZHANG Wei1, XIE Zhipeng1, LUO Wei2, ZHANG Jianguo1

Online:2011-05-05 Published:2011-05-05

摘要/Abstract

关键词: FONT-SIZE: 10.5pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>2-FONT-SIZE: 10.5pt, mso-ascii-font-family: Times New Roman, mso-hansi-font-family: Times New Roman, mso-bidi-font-family: Times New Roman, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">酮基FONT-SIZE: 10.5pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>-D-FONT-SIZE: 10.5pt, mso-ascii-font-family: Times New Roman, mso-hansi-font-family: Times New Roman, mso-bidi-font-family: Times New Roman, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">葡萄糖酸；发酵工艺；优化；动力学模型；FONT-SIZE: 10.5pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>Serratia sp.BK-98

Abstract:

Based on the optimization of culture conditions for producing 2-keto-D-gluconic acid(2-KDG)by Serratia sp.BK-98 in a Erlenmeyer flask, the factors of dissolved oxygen(DO)and pH affecting 2-KDG batch fermentation in 100 L fermenter were further optimized to be 30% and 6.0 respectively.Under the DO-stat and pH-stat batch culture conditions, 2-KDG production reached 211.2 g·L^-1.The kinetics of DO-stat and pH-stat batch fermentation were also investigated and the models for biomass, substrate consumption and product were established respectively based on the Logistic equation, Leudeking-Piret equation and Modified Leudeking-Piret equation.Curve fittings for the above models by using experimental data were performed by the non-linear least squares method with the software Origin 8.0.With the evaluated model parameters, the calculated values of the models and experimental data were in good agreement and the models could provide guidance for 2-KDG fermentation production.

Key words: FONT-SIZE: 10.5pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>2-FONT-SIZE: 10.5pt, mso-ascii-font-family: Times New Roman, mso-hansi-font-family: Times New Roman, mso-bidi-font-family: Times New Roman, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">酮基FONT-SIZE: 10.5pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>-D-FONT-SIZE: 10.5pt, mso-ascii-font-family: Times New Roman, mso-hansi-font-family: Times New Roman, mso-bidi-font-family: Times New Roman, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">葡萄糖酸；发酵工艺；优化；动力学模型；FONT-SIZE: 10.5pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>Serratia sp.BK-98

张炜, 谢志鹏, 罗玮, 张建国. 沙雷氏菌 sp.BK-98发酵生产2-酮基-D-葡萄糖酸的工艺优化及动力学研究 [J]. 化工学报, 2011, 62(5): 1371-1376.

ZHANG Wei, XIE Zhipeng, LUO Wei, ZHANG Jianguo. Fermentation process optimization and kinetics studies of 2-keto-D-gluconic acid production by Serratia sp.BK-98[J]. CIESC Journal, 2011, 62(5): 1371-1376.

[1]	任楠, 王涛, 吴玉庭, 马重芳. 混合碳酸盐的DSC测量与比热容分析 [J]. 化工学报, 2011, 62(S1): 197-202.
[2]	吴海波, 方岩雄, 纪红兵. 助溶剂促进的β-环糊精与肉桂醛的包结性能 [J]. 化工学报, 2011, 62(S1): 168-173.
[3]	褚燕彬, 曹柳林, 王晶. 利用组合B样条神经网络实现对间歇反应过程的动态建模 [J]. 化工学报, 2011, 62(S1): 157-162.
[4]	曾庆, 郭印诚, 牛振祺, 林文漪. 填料塔中氨水吸收二氧化碳的传质性能 [J]. 化工学报, 2011, 62(S1): 146-150.
[5]	张艾萍, 谢立强, 徐志明. 超声空化对换热器协同场的影响 [J]. 化工学报, 2011, 62(S1): 130-133.
[6]	王建立, 朱建军, 宋辰兴, 张兴. 3ω法测量纳米流体热导率 [J]. 化工学报, 2011, 62(S1): 42-47.
[7]	马维刚, 王海东, 张兴, 王玮. 多晶金薄膜电子-声子耦合的实验研究 [J]. 化工学报, 2011, 62(S1): 48-53.
[8]	崔福庆, 何雅玲, 程泽东, 李东, 陶于兵. 有压腔式吸热器内辐射传播过程的Monte Carlo模拟 [J]. 化工学报, 2011, 62(S1): 60-65.
[9]	何奎, 汪双凤, 黄间珍. 气液两相流在微小T型三通中的相分配特性 [J]. 化工学报, 2011, 62(S1): 92-96.
[10]	刘绩伟, 吴玉庭, 马重芳. 管肋式空间辐射器设计 [J]. 化工学报, 2011, 62(S1): 103-107.
[11]	李旺, 王情愿, 李瑞龙, 李超, 宇波, 张劲军, 代鹏飞. 大型浮顶油罐温度场数值模拟 [J]. 化工学报, 2011, 62(S1): 108-112.
[12]	胡朝发, 贾力. 脉动热管气液塞振荡运动模型 [J]. 化工学报, 2011, 62(S1): 113-117.
[13]	彭冬根1，曹熔泉2,3，张小松2，殷勇高2. 基于热不平衡考虑的溶液除湿/再生传热传质系数(Ⅱ)实验与模型计算分析 [J]. CIESC Journal, 2011, 62(9): 2440-2446.
[14]	马淳安，储诚普，徐颖华，张虹. 3,6-二氯吡啶甲酸合成方法进展 [J]. CIESC Journal, 2011, 62(9): 2398-2405.
[15]	刘夺, 杜瑾, 赵广荣, 元英进. 合成生物学在医药及能源领域的应用 [J]. 化工学报, 2011, 62(9): 2391-2397.