关键词: 还原型辅酶Ⅱ, 辅酶工程, 代谢调控, 工业微生物

Abstract: Nicotinamide adenine dinucleotide phosphate (NADPH)，a hemoprotein reductase，or in older notation，triphosphopyridine nucleotide is one of the most abundant redox cofactors in the metabolic network. It is the main electron donor in anabolism and is mainly used in the biosynthesis of biomass precursors. As an important branch of metabolism engineering，cofactor engineering changes the NADPH regeneration pathways and rebuilds the components of metabolic products. In this paper，we introduce the pathways in which NADPH is generated and reviewed the strategies to operate NADPH，including overexpression of enzymes，deletion of genes associated with NADPH metabolism or introduction of heterogeneous NADPH metabolic pathways. More attention should be focused on deeper understanding of the interplay between the operation of NADPH and central carbon metabolic networks in the future.

Key words: NADPH, cofactor engineering, metabolic operation, industrial strain