关键词: S-腺苷蛋氨酸, 谷胱甘肽, ATP, 产朊假丝酵母, 联产发酵

Abstract: The concentrations of intracellular co-factors,such as ATP,ADP,NADH and NAD⁺ vital for energy metabolism within Candida utilis CCTCC M 209298 were measured by HPLC.Based on the levels of co-factors,especially ATP during batch cultivation of C.utilis CCTCC M 209298,we developed a novel culture strategy of L-cysteine addition followed by ATP accumulation(21 h) instead of cell growth ceased(15 h) for further enhanced co-production of S-adenosylmethionine and glutathione using energy metabolic analysis.Subsequently,we combined this strategy with high-cell-density culture of C.utilis CCTCC M 209298,and both high biomass and high co-production of S-adenosylmethionine and glutathione were achieved.The culture strategy under the guidance of ATP concentration was proved to be efficient in terms of full utilization of intracellular residual ATP,together with improvement of co-production of S-adenosylmethionine and glutathione,and the co-production of S-adenosylmethionine and glutathione attained 584.7 mg·L^-1 and 1004.4 mg·L^-1 from batch and fed-batch fermentation,which were 27.1% and 26.4% higher than the controls,respectively.The results presented in this study provide a feasible strategy for optimization of fermentative processes for enhancing the co-production of analogous useful chemicals biosynthesized by consuming energy.

Key words: S-adenosylmethionine, glutathione, ATP, Candida utilis, co-production