黑曲霉生产柠檬酸的控制论模型

doi:10.11949/j.issn.0438-1157.20141879

化工学报 ›› 2015, Vol. 66 ›› Issue (11): 4534-4539.DOI: 10.11949/j.issn.0438-1157.20141879

黑曲霉生产柠檬酸的控制论模型

韩玲玉¹, 成宝琨¹, 郑小梅², 孙际宾², 袁景淇¹

1 上海交通大学自动化系, 系统控制与信息处理教育部重点实验室, 上海 200240;
2 中国科学院天津工业生物技术研究所, 天津 300308

收稿日期:2014-12-18 修回日期:2015-08-09 出版日期:2015-11-05 发布日期:2015-11-05
通讯作者: 袁景淇
基金资助:
中国科学院系统微生物工程重点实验室开放课题;博士学科点专项科研基金项目(20110073110018)。

Cybernetic modeling for citric acid fermentation by Aspergillus niger

HAN Lingyu¹, CHENG Baokun¹, ZHENG Xiaomei², SUN Jibin², YUAN Jingqi¹

1 Department of Automation, Shanghai Jiao Tong University, Shanghai 200240, China;
2 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China

Received:2014-12-18 Revised:2015-08-09 Online:2015-11-05 Published:2015-11-05
Supported by:
supported by the Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology/ Chinese Academy of Science and the Doctoral Program of Higher Education (20110073110018).

摘要/Abstract

摘要：

黑曲霉发酵是工业生产柠檬酸的主要途径,研究该过程的动力学模型对更好地理解这一工业微生物的代谢机理有重要意义。基于黑曲霉(Aspergillus niger)柠檬酸发酵过程主要代谢途径的分析,构建了简化的碳代谢网络,建立了胞内物质和酶浓度的平衡模型。引入一阶闭环调节器模型描述发酵初期微生物酶系的建立过程。结合生物反应器模型,给出了重要宏观状态变量(如细胞物质浓度、基质浓度、产物浓度)和胞内酶浓度的动态仿真结果与实验值的对比。

关键词: 黑曲霉, 柠檬酸, 控制论, 动力学模型, 胞内酶浓度, 代谢仿真

Abstract:

Aspergillus niger fermentation is the main way for the industrial production of citric acid. Modeling of Aspergillus niger from the perspective of cybernetics is significant for better understanding the metabolic behavior of this industrial microorganism. The metabolic pathways in Aspergillus niger during citric acid fermentation are investigated. Based on the simplified metabolic network, a cybernetic model describing the balance of intracellular metabolites and enzyme levels is constructed. Meanwhile, a first-order closed-loop regulator model is used to describe the transient enzyme pool establishment shortly after the inoculation. Combining with the bioreactor model, it is found that model simulations of intracellular enzyme concentrations are in coincidence with the measurements qualitatively, while the macroscopic state variables (biomass concentration, substrate concentrate and product concentrations) agree well with the offline assays.

Key words: Aspergillus niger, citric acid, cybernetic, kinetic model, intracellular enzyme concentration, metabolism simulation

中图分类号:

TP273

韩玲玉, 成宝琨, 郑小梅, 孙际宾, 袁景淇. 黑曲霉生产柠檬酸的控制论模型[J]. 化工学报, 2015, 66(11): 4534-4539.

HAN Lingyu, CHENG Baokun, ZHENG Xiaomei, SUN Jibin, YUAN Jingqi. Cybernetic modeling for citric acid fermentation by Aspergillus niger[J]. CIESC Journal, 2015, 66(11): 4534-4539.

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黑曲霉生产柠檬酸的控制论模型

Cybernetic modeling for citric acid fermentation by Aspergillus niger

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