AOAO-SNEDPR系统强化内源反硝化除磷研究

doi:10.11949/0438-1157.20250648

摘要/Abstract

摘要：

构建了厌氧/好氧/缺氧/好氧同步硝化内源反硝化除磷（AOAO-SNEDPR）系统，考察了不同进水磷浓度条件下脱氮除磷效果。在运行周期为6 h，进水COD、 $N H 4 +$ -N浓度分别为312.05 mg/L和52.37 mg/L时，梯度提高进水 $P O 4 3 -$ -P由3 mg/L至14.94 mg/L，系统出水稳定，COD、TN和 $P O 4 3 -$ -P平均浓度分别为30.47、7.14和0.31 mg/L。系统脱氮主要是基于好氧段内源反硝化以及缺氧段反硝化除磷而实现的，除磷基于好氧吸磷和反硝化吸磷。随着进水 $P O 4 3 -$ -P浓度提高，缺氧段TN和 $P O 4 3 -$ -P去除量占整个反应阶段去除量的比例分别提高至17.39%和7.85%。在高磷条件下，厌氧段内碳源储存率达到93.84%，聚磷菌（PAOs）和聚糖菌（GAOs）对内碳源储存的贡献约为67.14%和32.86%；反硝化聚磷菌（DPAOs）Dechloromonas和Candidatus_Accumulibacter的丰度分别达到2.69%和2.49%，反硝化聚糖菌（DGAOs）Candidatus_Competibacter菌群丰度达到3.34%。

关键词: SNEDPR, 内源反硝化, 反硝化除磷, 反硝化聚糖菌, 反硝化聚磷菌

Abstract:

In this study, the anaerobic/aerobic/anoxic/aerobic simultaneous nitrification, endogenous denitrification, and phosphorus removal (AOAO-SNEDPR) system was constructed to investigate the nitrogen and phosphorus removal performance under varying influent phosphorus concentrations. By combining measurements of sludge denitrifying phosphorus removal activity and microbial community structure analysis, the mechanisms of enhanced nitrogen and phosphorus removal via denitrifying phosphorus removal were elucidated. The experimental results demonstrated that under a 6 h operational cycle with influent COD and $N H 4 +$ -N concentrations of 312.05 mg/L and 52.37 mg/L, respectively, the system maintained stable treatment efficiency as the influent $P O 4 3 -$ -P concentration was incrementally increased from 3 to 14.94 mg/L. The average effluent concentrations of COD, TN, and $P O 4 3 -$ -P were 33.47, 7.14 and 0.31 mg/L, respectively. Nitrogen removal was primarily achieved through exogenous denitrification in the anaerobic phase, endogenous denitrification in the aerobic phase, and denitrifying phosphorus removal in the anoxic phase, while phosphorus removal was accomplished via aerobic phosphorus uptake and denitrifying phosphorus uptake. As the influent $P O 4 3 -$ -P concentration increased, the proportions of TN and $P O 4 3 -$ -P removal in the anoxic phase relative to the total removal across all phases rose to 17.39% and 7.85%, respectively. Under high phosphorus conditions, the average carbon storage rate in the anaerobic phase reached 93.84%, with polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs) contributing approximately 67.14% and 32.86% to the internal carbon storage, respectively. The abundances of the denitrifying phosphate-accumulating organisms (DPAOs) Dechloromonas and Candidatus_Accumulibacter reached 2.69% and 2.49%, respectively, while the abundance of the denitrifying glycogen-accumulating organism (DGAOs) Candidatus_Competibacter reached 3.34%.

Key words: SNEDPR, endogenous denitrification, denitrifying phosphorus removal, DGAOs, DPAOs

中图分类号:

X 703.1

贾一凡, 郭海燕, 任柯睿, 关典勇, 李玉明. AOAO-SNEDPR系统强化内源反硝化除磷研究[J]. 化工学报, 2025, 76(12): 6669-6679.

Yifan JIA, Haiyan GUO, Kerui REN, Dianyong GUAN, Yuming LI. Enhanced endogenous denitrifying phosphorus removal in AOAO-SNEDPR system[J]. CIESC Journal, 2025, 76(12): 6669-6679.

图/表 11

图1 SBR实验装置

Fig.1 Experimental setup of the SBR

表1 不同实验阶段反应器进水水质

Table 1 Influent wastewater quality of the reactor during different experimental phases

阶段	$N H 4 +$ -N/(mg/L)	COD/(mg/L)	$P O 4 3 -$ -P/(mg/L)	C/P
Ⅰ（1~21 d）	52.37	312.05	3.07	100
Ⅱ（22~57 d）	53.11	322.13	6.17	50
Ⅲ（58~79 d）	50.46	319.84	9.15	33
Ⅳ（80~119 d）	51.65	308.85	14.94	20

表1 不同实验阶段反应器进水水质

Table 1 Influent wastewater quality of the reactor during different experimental phases

阶段	$N H 4 +$ -N/(mg/L)	COD/(mg/L)	$P O 4 3 -$ -P/(mg/L)	C/P
Ⅰ（1~21 d）	52.37	312.05	3.07	100
Ⅱ（22~57 d）	53.11	322.13	6.17	50
Ⅲ（58~79 d）	50.46	319.84	9.15	33
Ⅳ（80~119 d）	51.65	308.85	14.94	20

图2 COD浓度及其去除率变化

Fig.2 Changes in COD concentration and removal efficiency

图3 PO43--P浓度及其去除率变化

Fig.3 Changes in PO43--P concentration and removal efficiency

图4 各态氮浓度及TN去除率变化

Fig.4 Changes in nitrogen concentration and TN removal efficiency

图5 不同进水PO43--P浓度条件下典型周期反应器内PO43--P浓度和COD变化（图例中P-3、P-9、P-15分别代表进水PO43--P浓度为3、9、15 mg/L工况）

Fig.5 Variations of PO43--P and COD concentrations in the reactor during typical cycles under different influent PO43--P concentrations

图6 不同进水PO43--P浓度条件下典型周期反应器内各态氮和TN变化情况

Fig.6 Variations of nitrogen species and TN in the reactor during typical cycles under different influent PO43--P concentrations

图7 典型周期反应器内DO、ORP和pH变化情况

Fig.7 Variations of DO, ORP and pH in the reactor during typical cycles

图8 典型周期反应器污泥内碳源转化情况

Fig.8 Carbon source transformation in sludge during typical cycles of the reactor

图9 不同电子受体DGAOs和DPAOs活性测定

Fig.9 Activity determination of DGAOs and DPAOs under different electron acceptor conditions

图10 微生物菌群结构变化

Fig.10 Variations in microbial community structure of the sludge

参考文献 30

[1]	Luo Y H, Yi K, Zhang X Y, et al. Simultaneous partial nitrification, denitrification, and phosphorus removal in sequencing batch reactors via controlled reduced aeration and short-term sludge retention time decrease[J]. Journal of Environmental Management, 2023, 344: 118598.
[2]	戴娴, 王晓霞, 彭永臻, 等. 进水C/N对富集聚磷菌的SNDPR系统脱氮除磷的影响[J]. 中国环境科学, 2015, 35(9): 2636-2643.
	Dai X, Wang X X, Peng Y Z, et al. Effect of influent C/N ratio on nitrogen and phosphorus removal in an SNDPR system enriched with polyphosphate accumulating organisms[J]. China Environmental Science, 2015, 35(9): 2636-2643.
[3]	Campo R, Sguanci S, Caffaz S, et al. Efficient carbon, nitrogen and phosphorus removal from low C/N real domestic wastewater with aerobic granular sludge[J]. Bioresource Technology, 2020, 305: 122961.
[4]	Huang X, Zhu J, Duan W Y, et al. Biological nitrogen removal and metabolic characteristics in a full-scale two-staged anoxic-oxic (A/O) system to treat optoelectronic wastewater[J]. Bioresource Technology, 2020, 300: 122595.
[5]	Wang X X, Wang S Y, Zhao J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR)[J]. Water Research, 2016, 95: 319-329.
[6]	Yuan C S, Wang B, Peng Y Z, et al. Enhanced nutrient removal of simultaneous partial nitrification, denitrification and phosphorus removal (SPNDPR) in a single-stage anaerobic/micro-aerobic sequencing batch reactor for treating real sewage with low carbon/nitrogen[J]. Chemosphere, 2020, 257: 127097.
[7]	Wang X X, Zhao J, Yu D S, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019, 355: 560-571.
[8]	戴娴, 彭永臻, 王晓霞, 等. 不同厌氧时间对富集聚磷菌的SNDPR系统处理性能的影响[J]. 中国环境科学, 2016, 36(1): 92-99.
	Dai X, Peng Y Z, Wang X X, et al. Effect of different anaerobic durations on the performance of an SNDPR system enriched with polyphosphate-accumulating organisms[J]. China Environmental Science, 2016, 36(1): 92-99.
[9]	王晓霞, 王淑莹, 赵骥, 等. 厌氧/好氧SNEDPR系统处理低C/N比污水的优化运行[J]. 中国环境科学, 2016, 36(9): 2672-2680.
	Wang X X, Wang S Y, Zhao J, et al. Optimized operation of an anaerobic/aerobic SNEDPR system for low C/N ratio wastewater treatment[J]. China Environmental Science, 2016, 36(9): 2672-2680.
[10]	王晓霞, 王淑莹, 赵骥, 等. SPNED-PR系统内PAOs-GAOs的竞争关系及其氮磷去除特性[J]. 中国环境科学, 2018, 38(2): 551-559.
	Wang X X, Wang S Y, Zhao J, et al. Competitive relationship between PAOs and GAOs in SPNED-PR system and its nitrogen and phosphorus removal characteristics[J]. China Environmental Science, 2018, 38(2): 551-559.
[11]	任丽芳, 巩有奎, 孙洪伟. 碳氮比对AOA-SBR同步脱氮除磷性能及N₂O释放的影响[J]. 环境工程, 2024, 42(5): 1-9.
	Ren L F, Gong Y K, Sun H W. Effects of carbon to nitrogen ratio on simultaneous nitrogen and phosphorus removal performance and N₂O emission in AOA-SBR system[J]. Environmental Engineering, 2024, 42(5): 1-9.
[12]	张杰, 杨杰, 李冬, 等. AOA-O模式下好氧颗粒污泥同步硝化内源反硝化除磷[J]. 中国环境科学, 2023, 43(10): 5226-5234.
	Zhang J, Yang J, Li D, et al. Simultaneous nitrification, endogenous denitrification and phosphorus removal by aerobic granular sludge under AOA-O mode[J]. China Environmental Science, 2023, 43(10): 5226-5234.
[13]	国家环境保护总局. 水和废水监测分析方法[M]. 4版. 北京: 中国环境科学出版社, 2002.
	State Environmental Protection Administration (SEPA). Water and Wastewater Monitoring and Analysis Methods [M]. 4th ed. Beijing: China Environmental Science Press, 2002.
[14]	刘俊梅, 王璐, 李琢伟, 等. 产聚β-羟基丁酸酯(PHB)菌种的筛选及16S rDNA鉴定[J]. 粮油加工, 2014, 4(17): 71-75.
	Liu J M, Wang L, Li Z W, et al. Screening and 16S rDNA identification of p o l y - β - h y d r o x y b u t y r a t e (PHB)-producing bacterial strains[J]. Cereals and Oils Processing, 2014, 4(17): 71-75.
[15]	Luan Y N, Yin Y, Guo Z H, et al. Achieving simultaneous nitrification and endogenous denitrifying phosphorus removal in anaerobic/intermittently-aerated moving bed biofilm reactor for low carbon-to-nitrogen ratio wastewater treatment[J]. Bioresource Technology, 2024, 394: 130178.
[16]	Wang X X, Wang S Y, Xue T L, et al. Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015, 77: 191-200.
[17]	都叶奇, 于德爽, 甄建园, 等. 进水C/N对SNEDPR系统脱氮除磷的影响[J]. 环境科学, 2019, 40(2): 816-822.
	Du Y Q, Yu D S, Zhen J Y, et al. Effect of influent C/N ratio on nitrogen and phosphorus removal in SNEDPR system[J]. Environmental Science, 2019, 40(2): 816-822.
[18]	李冬, 李悦, 李雨朦, 等. 好氧颗粒污泥同步硝化内源反硝化脱氮除磷[J]. 中国环境科学, 2022, 42(3): 1113-1119.
	Li D, Li Y, Li Y M, et al. Simultaneous nitrification, endogenous denitrification and phosphorus removal by aerobic granular sludge[J]. China Environmental Science, 2022, 42(3): 1113-1119.
[19]	韩芸, 许松, 董涛, 等. 碳源类型、温度及电子受体对生物除磷的影响[J]. 环境科学, 2015, 36(2): 590-596.
	Han Y, Xu S, Dong T, et al. Effects of carbon source type, temperature and electron acceptor on biological phosphorus removal[J]. Environmental Science, 2015, 36(2): 590-596.
[20]	Hou H H, Duan L, Zhou B H, et al. The performance and degradation mechanism of sulfamethazine from wastewater using IFAS-MBR[J]. Chinese Chemical Letters, 2020, 31(2): 543-546.
[21]	Chen P, Wu J K, Lu X W, et al. Denitrifying phosphorus removal and microbial community characteristics of two-sludge DEPHANOX system: effects of COD/TP ratio[J]. Biochemical Engineering Journal, 2021, 172: 108059.
[22]	Wang R F, Yang C Z, Hu H, et al. The impact of the varying nutrient concentrations on the enhanced biological phosphorus removal performance and functional phosphorus-accumulating and denitrifying genes in an anaerobic-aerobic-anoxic sequencing batch reactor[J]. Environmental Technology & Innovation, 2021, 21: 101256.
[23]	王亚东, 王少坡, 郑莎莎, 等. 生物除磷系统的聚磷微生物种群及其检测方法[J]. 环境工程, 2015, 33(2): 21-26.
	Wang Y D, Wang S P, Zheng S S, et al. Polyphosphate-accumulating organisms populations and their detection methods in biological phosphorus removal systems[J]. Environmental Engineering, 2015, 33(2): 21-26.
[24]	刘旭, 王继华, 车琦, 等. AOA-SBR系统运行效能及高效聚磷菌的特性研究[J]. 环境科学研究, 2019, 32(8): 1427-1436.
	Liu X, Wang J H, Che Q, et al. Operational performance of AOA-SBR system and characteristics of high-efficiency phosphate-accumulating organisms[J]. Research of Environmental Sciences, 2019, 32(8): 1427-1436.
[25]	Nittami T, Kasakura R, Kobayashi T, et al. Exploring the operating factors controlling Kouleothrix (type 1851), the dominant filamentous bacterial population, in a full-scale A2O plant[J]. Scientific Reports, 2020, 10: 6809.
[26]	王丽谦. 低碳市政污水好氧颗粒污泥培养及脱氮除磷性能研究[D]. 北京: 北京化工大学, 2020.
	Wang L Q. Cultivation of aerobic granular sludge and its nitrogen & phosphorus removal performance in low-carbon municipal wastewater treatment[D]. Beijing: Beijing University of Chemical Technology, 2020.
[27]	He Q L, Chen L, Zhang S J, et al. Hydrodynamic shear force shaped the microbial community and function in the aerobic granular sequencing batch reactors for low carbon to nitrogen (C/N) municipal wastewater treatment[J]. Bioresource Technology, 2019, 271: 48-58.
[28]	Xu X C, Qiu L Y, Wang C, et al. Achieving mainstream nitrogen and phosphorus removal through simultaneous partial nitrification, anammox, denitrification, and denitrifying phosphorus removal (SNADPR) process in a single-tank integrative reactor[J]. Bioresource Technology, 2019, 284: 80-89.
[29]	李冬, 高鑫, 张杰, 等. 长/短HRT交替培养同步硝化反硝化除磷颗粒[J]. 哈尔滨工业大学学报, 2023, 55(2): 9-18.
	Li D, Gao X, Zhang J, et al. Alternating long/short HRT cultivation for simultaneous nitrification, denitrification and phosphorus removal granules[J]. Journal of Harbin Institute of Technology, 2023, 55(2): 9-18.
[30]	Fan Z W, Zeng W, Wang B G, et al. Transcriptional responses of Candidatus Accumulibacter clades to environmental dynamics in enhanced biological phosphorus removal[J]. Bioresource Technology, 2020, 306: 123108.