Abstract: The technical aspects of a new and powerful separation technique, extractive purification by aqueous two-phase systems were studied in detail. According to the characteristics of aqueous two phase systems, a φ20 cylindrical centrifugal extractor was selected as the equipment for extractive purification of α-amylase. The hydraulic characteristics of the installation and the effects of speed and total throughput on the yield and Murphree efficiency of α-amylase extraction were investigated. A speed of 3380-3800r/min, a flow ratio of 0.2-0.5 and a total throughput of 25-50ml/min were found as the optimal operation range for while 90% of phases purity, 75% yield and 90% Murphree efficiency for a-amylase could be obtained.

摘要： 根据双水相体系的特点,选择了Ф20(环)圆筒式离心萃取器进行a-淀粉酶的双水相体系萃取实验,考察了萃取器的水力学特性,确定了萃取操作范围,并考察了萃取器转鼓转速、总流量对体系传质特性、a-淀粉酶失活和收率的影响.结果表明,在转速3380～3800r/min、流比0.2～0.5、总流量25～50ml/min范围内,萃取器的轻相夹带量可在10%以内,重相夹带量趋近于零,a-淀粉酶的失活可以忽略不计,萃取效率和收率分别达90%和75%以上.