Abstract: A systematic approach for predicting the hindrance factor of macromolecular diffusion in micropores is reported, simultaneously taking into account the molecular dimension and shape effects. The general theoretical fundamentals are described in term of the movement of a rigid spherical particle through a long circular cylindrical pore of comparable dimension. Modeled as one of six typical shapes, the shape effect of macromolecules is considered by defining a geometric characteristic radius to calculate the concentration partitioning coefficient and a hydrodynamic equivalent sphere radius to evaluate the hydrodynamic reverse drag coefficient, in substitution of the previously used Stokes -Einstein radius. The experimental tests for four macromolecules of typical shapes: dextrane, ficoll, polystyrene and bovine serum albumin, are carried out with the polycarbonate track-etched membranes. The quantitative comparison indicates that the results obtained from the theoretical calculation are consistent with the experimental data.

摘要： 研究了大分子在微孔中的阻滞扩散,描述一种同时计算分子尺寸和形状对阻滞效果影响的方法,并使用聚碳酸酯核径迹膜对4种典型形状的大分子进行了实验研究.