Abstract: The removal of yeast cell debris following high - speed bead mill disruption was studied by using polyethylene glycol (PEG) precipitation. The partition rule of cell debris was investigated in PEG/inorganic salts systems. Cell debris might be partitioned between the bottom, intermediate and top phases in different aqueous two - phase compositions. When there was only PEG or there were PEG and a little amount of salt, yeast homogenate was separated into a supernatant containing intracellular products and a solid cell debris sediment by using only low speed centrifugation. The effective removal of cell debris and the extraction of intracellular alcohol dehydrogenase were further studied by using the liquid -solid two -phase system and aqueous two -phase systems.

摘要： <文> 自 80年代以来，双水相苯取技术用于生化产品的分离受到重视，尤其对聚乙二醇 （PEG）和无机盐体系的开发研究更多“，“，双水相荤取技术在分离细胞匀浆中胞内产物和细胞 碎片方面，显示出可取代高速离心和微孔膜过滤的潜力。目前大多数研究集中在双水相革取的 机理和分离纯化不同蛋白质上，对清除细胞碎片的应用研究尚不多见。本文从细胞碎片在 PEG－无机盐系统的分配规律中发规系统未形成双水相前，存在一个液－固两相生成区。在此 区内大部分碎片形成固相沉淀，低速离心即可除去。以从酵母中提取醇脱氢酶为例，将这种新 的售脸碰计茉D体同Nil。t。十签人（门的W＊栅茉阶体淋杆了计林