Abstract: A combined system of immobilized enzyme reactor and ion exchange column was employed for hydrolysis of Penicillin G to 6 -Aminopenicillanic acid with continuous removal of Phenylacetic acid . In the composite system, the time required for 95% Penicillin G conversion to 6 -Aminopenicillanic acid could be reduced by 10. 8% and 53% of Phenylacetic acid could be removed from the reaction mixture. Based on Phenylacetic acid and Penicillin G dynamics in ion exchange process and immobilized enzyme kinetics, the hydrolysis of Penicillin G was simulated well by computer from the mathematical model developed.

摘要： 采用固定化青霉素酰化酶反应器进行青霉素G水解生产6-氨基青霉烷酸,同时与离子交换柱相组合以连续地去除苯乙酸而维持反应混合液中的低苯乙酸浓度.研究发现,采用随程离子交换法可提高青霉素G水解速率10.2%,同时可从反应混合液中去除53%的苯乙酸;通过确定苯乙酸、青霉素G在离子交换柱中的动力学行为和固定化酶的动力学参数,利用已建立的数学模型,可很好地对青霉素G水解生产6-氨基青霉烷酸过程进行计算机模拟.