Abstract: The effects of ultrasonic treatment on the microbial hydyoxylation transformation of steroid substrate were investigated. Ultrasonic irradiation with comparatively low power and low frequency displayed its notable capability of handling a higher content of steroid substrate for biotransformation. Its function may mainly come from two sides, one is its mechanical action to make the solid substrate finer by interat-trition of those suspended particles, the other is due to its cavitation effect to facilitate easier passage of the fine substrate through the cellular membrane.

摘要： <正>超声应用于生物技术有其独到之处。超声波是一种波动形式,同时也是一种能量传递形式。利用超声辐照的机械作用以及控制适当的空化作用改善传质和调变膜透性等效应在生物技术中具有重要应用价值。有关超声技术应用于甾体微生物转化体系的研究已有一些报道,但大多集中在对甾体底物悬浮液的处理方面,尚未涉及对生物体系自身的辐照与激活。本文以超声在甾体11α羟化反应中的应用为背景,考察了超声因子、超声方式和时间、超声与生长调节剂的配合使用等因素对超声效应的影响。