Abstract: Sodium bis (2 - ethylhexyl polyoxyalkylene) sulfosuccinates (SEPES) were prepared and used to form the reversed micelles in isooctane. Taking the hydrolysis of olive oil as a model reaction, the effects of average polymerization number ( n) of ethylene oxide and water content on the activity and stability of lipase in the reversed micelles were investigated. It was found that the activity, the apparent turnover number, and the deactivation rate coefficient of lipase in the reversed micelles formed by SEPES at n=2.0 in isooctane were respectively 2.2, 2.1 and 0.39 times, more than those in AOT/isooctane reversed micelles, while the apparent Michaelis constants of lipase in the two systems were of little differ-

摘要： <正>反胶团酶反应兼有水相与有机相酶反应的共同优点,但至今尚无工业应用的重要原因是反胶团中酶的活性和稳定性不高.常用的离子型表面活性剂,如二-(2-乙基己基)琥珀酸双酯磺酸钠(AOT)在有机溶剂中形成的反胶团,因相界面与酶分子间的静电作用将造成酶在反胶团中迅速失活.而非离子型表面活性剂形成的反胶团中,酶一般具有较高的活性和稳定性,但这种体系需加入助表面活性剂,给下游加工过程带来麻烦.作者制备了具有不同氧乙烯聚合数(n)的二-(2-乙基己基聚氧乙烯)琥珀酸双酯磺酸钠(简称SEPES),其分子结构与AOT相似,但其极性头与疏水尾间连有聚氧乙烯链非离子性亲水基团.本文报道脂肪酶在SEPES/异辛烷反胶团体系中的特性.