Abstract: A new kind of electrophoretic affinity chromatography (EAC) for bioseparation was
proposed,Separation by EAC was conducted in a multicompartment electrolyzer in which the
affinity gel media were packed in one of the central compartments.The presence of an
electric field accelerated the migration of proteins inside the gel matrix during
adsorption and descrption processes,This led to the increase of the overall speed of
separation,The present study was focused on the effect of the strength of the electric
field on adsorption and desorption processes.

Key words: affinity chromatography, electrophoresis, electrophoretic affinity chromatography, human serum albumin, Cibacron Blue F3G-A

摘要： A new kind of electrophoretic affinity chromatography (EAC) for bioseparation was
proposed,Separation by EAC was conducted in a multicompartment electrolyzer in which the
affinity gel media were packed in one of the central compartments.The presence of an
electric field accelerated the migration of proteins inside the gel matrix during
adsorption and descrption processes,This led to the increase of the overall speed of
separation,The present study was focused on the effect of the strength of the electric
field on adsorption and desorption processes.

关键词: 生物分离;蛋白质;电泳亲合色谱法;EAC;多组分电解剂;吸附;解吸附