Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity  Partitioning

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Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity
Partitioning

LIN Dongqiang; YAO Shanjing; MEI Lehe; ZHU Ziqiang

Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027,
China

Received:1900-01-01 Revised:1900-01-01 Online:2000-12-18 Published:2000-12-18
Contact: LIN Dongqiang

固定化金属离子亲和分配用的亚胺二乙酸-聚乙二醇偶联物的制备

林东强; 姚善泾; 梅乐和; 朱自强

Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027,
China

通讯作者: 林东强

Abstract

Abstract: The synthesis route was investigated and optimized for the preparation of iminodiacetic
acid polyethylene glycol (IDA-PEG) for immobilized metal ion affinity partitioning in
aqueous two-phaze systems. IDA PEG was synthesized from PEG in two steps by the reaction of
iminodiacetic acid with a monosubetituted derivative of epichlorohydrin-activated PEG. The
Cu2+ content combined with IDA-PEG was determined by atomic absorp tion spectrometry as 0.5
mol.mol-1 (PEG). Furthermore, the affinity partitioning behavior of lactate dehydrogenase
in polyethylene glycol/hydroxypropyl starch aqueous two-phaze systems was studied to
clarify the affinity effect of the Cu(Ⅱ)-IDA-PEG.

Key words: immobilized metal ion affinity partitioning, iminodiacetic acid-polyethylene glycol, aqueous two-phase system

摘要： The synthesis route was investigated and optimized for the preparation of iminodiacetic
acid polyethylene glycol (IDA-PEG) for immobilized metal ion affinity partitioning in
aqueous two-phaze systems. IDA PEG was synthesized from PEG in two steps by the reaction of
iminodiacetic acid with a monosubetituted derivative of epichlorohydrin-activated PEG. The
Cu2+ content combined with IDA-PEG was determined by atomic absorp tion spectrometry as 0.5
mol.mol-1 (PEG). Furthermore, the affinity partitioning behavior of lactate dehydrogenase
in polyethylene glycol/hydroxypropyl starch aqueous two-phaze systems was studied to
clarify the affinity effect of the Cu(Ⅱ)-IDA-PEG.

关键词: immobilized metal ion affinity partitioning;iminodiacetic acid-polyethylene glycol; aqueous two-phase system

LIN Dongqiang, YAO Shanjing, MEI Lehe, ZHU Ziqiang. Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity
Partitioning[J]. .

林东强, 姚善泾, 梅乐和, 朱自强. 固定化金属离子亲和分配用的亚胺二乙酸-聚乙二醇偶联物的制备[J]. CIESC Journal.

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