Abstract: The synthesis process of a biomimetic membrane directed by macromolecule(CTAB) assemblies was researched by using hydrolysis reaction of tetraethylorthosilicate. Influences of CTAB assembly configuration,pH values,initial concentrations of CTAB were investigated on the biomimetic membrane through characterizing techniques such as SEM and TEM.Integral and resilient freestanding membranes were synthesized under the optimized biomimetic operation conditions. These research results are essential to synthesise of high performance separation membranes through biomimetic methods.