Abstract: The procedure of enzymatic aqueous extraction of soybean oil was assessed when two-step
controlled enzymatic hydrolysis was applied. With aqueous extraction of soybean oil-
containing protein, the highest yield of oil was 96.1% at the optimized conditions studied.
Soybean oil-containing protein was hydrolyzed and resulted in releasing part of oil. The
separated protein that contained 40% oil was enriched due to its adsorption capacity of
released oil, the average oil extraction yeild reached 93.5%. Then the high oil content
protein was hydrolyzed again to release oil by enzyme, the oil extraction yeild was 80.4%.
As a result, high quality of soybean oil was obtained and the content of total oil yield was
74.4%.

Key words: soybean oil, aqueous extraction, enzymatic methods

摘要： The procedure of enzymatic aqueous extraction of soybean oil was assessed when two-step
controlled enzymatic hydrolysis was applied. With aqueous extraction of soybean oil-
containing protein, the highest yield of oil was 96.1% at the optimized conditions studied.
Soybean oil-containing protein was hydrolyzed and resulted in releasing part of oil. The
separated protein that contained 40% oil was enriched due to its adsorption capacity of
released oil, the average oil extraction yeild reached 93.5%. Then the high oil content
protein was hydrolyzed again to release oil by enzyme, the oil extraction yeild was 80.4%.
As a result, high quality of soybean oil was obtained and the content of total oil yield was
74.4%.

关键词: soybean oil;aqueous extraction;enzymatic methods