Calculation of Empirical and True Maintenance Coefficients by Flux Balance Analysis

• RESEARCH PAPERS • Previous Articles Next Articles

Calculation of Empirical and True Maintenance Coefficients by Flux Balance Analysis

Ma Hongwu; Zhao Xueming; Guo Xiaofeng

School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China

Received:1900-01-01 Revised:1900-01-01 Online:2002-02-28 Published:2002-02-28
Contact: Ma Hongwu

通过通量平衡分析计算经验和真实维持系数

马红武; 赵学明; 郭晓峰

School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China

通讯作者: 马红武

Abstract

Abstract: The stoichiometric matrix of a simplified metabolic network in Bacillus Subtillis was
constructed from the flux balance equations, which were used for reconciliation of the
measured rates and determination of the inner metabolic rates. Thus more reliable results
of the true and empirical maintenance coefficients were obtained. The true maintenance
coefficient is linearly related to the specific growth rate and changes with the P/O ratio.
The measured biomass yield of adenosine triphosphate(ATP) is also linearly related to the
P/O ratio.

Key words: maintenance coefficient, flux balance analysis, metabolic network, biomass yield

摘要： The stoichiometric matrix of a simplified metabolic network in Bacillus Subtillis was
constructed from the flux balance equations, which were used for reconciliation of the
measured rates and determination of the inner metabolic rates. Thus more reliable results
of the true and empirical maintenance coefficients were obtained. The true maintenance
coefficient is linearly related to the specific growth rate and changes with the P/O ratio.
The measured biomass yield of adenosine triphosphate(ATP) is also linearly related to the
P/O ratio.

关键词: maintenance coefficient;flux balance analysis;metabolic network;biomass yield

Ma Hongwu, Zhao Xueming, Guo Xiaofeng. Calculation of Empirical and True Maintenance Coefficients by Flux Balance Analysis[J]. .

马红武, 赵学明, 郭晓峰. 通过通量平衡分析计算经验和真实维持系数[J]. CIESC Journal.

References

[1] Ma, H. W., Zhao, X. M., Tang, Y. J., "Analysis of xanthan fermentation data in
stationary phase using black box model and metabolic network model", Chinese J. of
Chem.Eng., 7 (4), 321 (1999).
[2] Nielsen, J., Villadsen, J., Bioreaction Engineering Principles, Plenum Press, New York
(1994).
[3] Stephanopoulos, G., Aristidou, A., Nielsen, J., Metabolic Engineering, Academic Press,
San Diego (1998).
[4] Schilling, C. H., Schuster, S., Palsson, B. O., "Metabolic pathway analysis: basic
concepts and scientific applications in the post-genomic era", Biotechnol. Prog., 15 (3),
296(1999).
[5] Sauer, U., Hatzimanikatis, V., Hohmann, H., "Physiology and metabolic fluxes of wild-
type and riboflavin-producing Bacillus subtilis", Appl. Environ. Microbiol., 62 (10), 3687
(1996).
[6] Noorman, H. J., Romein, B., "Classification, error detection, and reconciliation of
process information in complex biochemical systems", Biotechnol. Bioeng., 49 (3), 364
(1996).
[7] Sauer, U., Bailey, J. E., "Estimation of P-to-O ratio in Bacillus subtillis and its
influence on maximum riboflavin yield", Biotechnol. Bioeng., 64 (6), 750 (1999).
[8] Stouthamer, A. H., Bettenhaussen, C., "Utilization of energy for growth and maintenance
in continuous and batch cultures of microorganisms: a reevaluation of the method for the
determination of the ATP production by measuring molar growth yields", Biochim. Biophys.
Acta, 301 (1), 53(1973).
[9] Nielsen, J., Physiological Engineering Aspects of Penicillum Chrysogenium, World
Scientific Publishing Co., Singapore (1997).
[10] Benthin, S., Schulze, U., Nielsen, J., Villadsen, J., "Growth energetics of
Lactococcus cremoris FD1 during energy-,carbon-, and nitrogen-limitation in steady state
and transient cultures", Chem. Eng. Science, 49 (5), 589 (1994).