Abstract: The activities of cellulase in 10%-40% ethanol- water solution were investigated at temperatures of 20-40 ℃ and at pressures of 0.49-4.41 MPa. These data obtained are essential to purify cellulase by high pressure CO₂. The experimental results indicate that CO2 pressure is not the key factor of the loss of enzyme activities, but ethanol concentration and temperature markedly influence recovered activities. The optimum operating conditions are 30 % ethanol concentration solution and 35 ℃ for cellulase- ethanol- water systems using high pressure CO₂.

摘要： 测定了10％-40％(质量)乙醇-水溶液中的纤维素酶在温度为20-40℃和CO₂压力为0.49-4.41MPa下的酶活变化数据,这些数据是用高压CO₂分离纯化纤维素酶的基础数据.实验表明,CO₂压力不是酶活损失的主要因素,乙醇浓度和温度对酶活损失的影响较大.在乙醇浓度小于30％、温度低于35℃的条件下,对纤维素酶-乙醇-水体系进行高压CO₂处理比较适宜.