Abstract: Human epidermal growth factor(hEGF),a polypeptide growth factor comprising of 53 amino acid residues, has become one of research hot spots in medical field because of its various biological functions. In order to purify hEGF, the influences of flow rate, sample volume and column height on separation performance of hEGF in gel chromatography are investigated. Results indicate that there is a little influence of flow rate on distribution coefficient of hEGF and distribution coefficient of hEGF apparently increases with the increase of sample volume injected, while column height has no influence on distribution coefficient of hEGF. The standard deviation of hEGF decreases with increasing flow rate and column height,especially when flow rate is less than 6cm·h ^-1 and column height is lower than 8cm. It also increases with the increase of sample volume injected,especially when the sample volume injected is larger than 3ml. These results imply that the resolution will sharply decrease when flow rate and column bed height are less than 6cm·h ^-1 and 8cm respectively and the sample volume injected is more than 3ml under given conditions.

摘要： 考察了流速、进样体积与柱床高度对人表皮生长因子 (human epidermal growth factor,hEGF)分离行为的影响 .结果表明 :在实验范围内 ,流速对hEGF的分配系数影响较小 ,hEGF的分配系数随样品体积的增加而上升 ,但柱床高度对hEGF的分配系数几乎无影响 .在流速小于 6cm·h^-1时 ,hEGF的标准偏差下降显著 ,而高流速对其影响较小 .当进样量小于 3ml时 ,hEGF的标准偏差上升较缓 ;当进样量大于 3ml时 ,hEGF的标准偏差显著上升 .在柱床高度小于 8cm时 ,hEGF的标准偏差下降较快 ;当柱床高度高于 8cm时 ,hEGF的标准偏差下降缓慢