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HIGH DENSITY FERMENTATION OF RECOMBINANT E.coli FOR PRODUCTION OF HUMAN-LIKE COLLAGEN

FAN Daidi;DUAN Mingrui;MI Yu;SONG Jirong;HUI Junfeng;WANG Dewei;WANG Guozhu   

  • Online:2002-07-25 Published:2002-07-25

重组E.coli工程菌高密度培养生产人源型胶原蛋白

范代娣; 段明瑞; 米钰; 宋纪蓉; 惠俊峰; 王德伟; 王国柱   

  1. 西北大学化工系

Abstract: Several technical parameters were studied during the fermentation of recombinant E.coli for the production of collagen-like biopolymer.The effects of dissolved oxygen as well as glucose concentration on fermentation were observed.The OD 600 value could reach 98 when dissolved oxygen was controlled at 50% and glucose around 1%.The production of human-like collagen with a yield of 29.4% was obtained.