ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3-  hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha

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ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3-
hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha

Longan SHANG^a,b; Jin Hwan DO^a; FAN Daidi^b; JIANG Min^a; Ho Nam CHANG^a

^a Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science
and Technology,373-1 Kusong-dong, Yusong-gu, Taejeon 305-701, Korea
^b Department of Chemical Engineering, Northwest University, Xi’an 710069, China

Received:1900-01-01 Revised:1900-01-01 Online:2003-04-28 Published:2003-04-28
Contact: Longan SHANG

Ralstonia eutropha批式流加培养生产羟基丁酸-丙酸混合聚酯中丙酸进料优化研究

Longan SHANG^a,b; Jin Hwan DO^a; 范代娣^b; 姜岷^a; Ho Nam CHANG^a

^a Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science
^{and Technology,373-1 Kusong-dong, Yusong-gu, Taejeon 305-701, Korea
b} Department of Chemical Engineering, Northwest University, Xi’an 710069, China

通讯作者: Longan SHANG

Abstract

Abstract: The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro
xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha was optimized to
achieve high cell density and high 3HV yield. Effects of different feeding strategies of
propionic acid on the production of P(3HB-co-3HV) were investigated. A decline of specific
synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due
to the propionic acid accumulation in culture broth when the feeding solution with high
P/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling
propionic acid concentration at a low level in the separate feeding of propionic acid. An
optimal feeding strategy was demonstrated to reduce the propionic acid accumulation. The
cell concentration, P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9 kg.m
-3,1.8 kg.m-3.h-1, and 10.6%(by mass), respectively, resulting in a yield of 0.33 g HV per
g propionic acid.

Key words: biodegradable polyester, feeding strategy, P(3HB-co-3HV), propionic acid, Ralstonia eutropha

摘要： The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro
xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha was optimized to
achieve high cell density and high 3HV yield. Effects of different feeding strategies of
propionic acid on the production of P(3HB-co-3HV) were investigated. A decline of specific
synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due
to the propionic acid accumulation in culture broth when the feeding solution with high
P/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling
propionic acid concentration at a low level in the separate feeding of propionic acid. An
optimal feeding strategy was demonstrated to reduce the propionic acid accumulation. The
cell concentration, P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9 kg.m
-3,1.8 kg.m-3.h-1, and 10.6%(by mass), respectively, resulting in a yield of 0.33 g HV per
g propionic acid.

关键词: biodegradable polyester;feeding strategy;P(3HB-co-3HV);propionic acid;Ralstonia eutropha

Longan SHANG, Jin Hwan DO, FAN Daidi, JIANG Min, Ho Nam CHANG. ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3-
hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha[J]. .

Longan SHANG, Jin Hwan DO, 范代娣, 姜岷, Ho Nam CHANG. Ralstonia eutropha批式流加培养生产羟基丁酸-丙酸混合聚酯中丙酸进料优化研究[J]. CIESC Journal.

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